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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F13%3A10209662%21RIV14-MSM-11130___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1089/scd.2012.0616
dcterms:description
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A suitable combination of modern hydrogel materials, modified to effectively bridge the lesion cavity, combined with appropriate stem cell therapy seems to be a promising approach to repair spinal cord damage. We demonstrate the synergic effect of porosity and surface modification of hydrogels on mesenchymal stem cell (MSC) adhesiveness in vitro and their in vivo survival in an experimental model of SCI. MSCs were seeded on four different hydrogels: hydroxypropylmethacrylate-RGD prepared by heterophase separation (HPMA-HS-RGD) and three other hydrogels polymerized in the presence of a solid porogen: HPMA-SP, HPMA-SP-RGD, and hydroxy ethyl methacrylate [2-(methacryloyloxy)ethyl] trimethylammonium chloride (HEMA-MOETACl). Their adhesion capability and cell survival were evaluated at 1, 7, and 14 days after the seeding of MSCs on the hydrogel scaffolds. The cell-polymer scaffolds were then implanted into hemisected rat spinal cord, and MSC survival in vivo and the ingrowth of endogenous tissue elements were evaluated 1 month after implantation. In vitro data demonstrated that HEMA-MOETACl and HPMA-SP-RGD hydrogels were superior in the number of cells attached. In vivo, the highest cell survival was found in the HEMA-MOETACl hydrogels; however, only a small ingrowth of blood vessels and axons was observed. Both HPMA-SP and HPMA-SP-RGD hydrogels showed better survival of MSCs compared with the HPMA-HS-RGD hydrogel. Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A suitable combination of modern hydrogel materials, modified to effectively bridge the lesion cavity, combined with appropriate stem cell therapy seems to be a promising approach to repair spinal cord damage. We demonstrate the synergic effect of porosity and surface modification of hydrogels on mesenchymal stem cell (MSC) adhesiveness in vitro and their in vivo survival in an experimental model of SCI. MSCs were seeded on four different hydrogels: hydroxypropylmethacrylate-RGD prepared by heterophase separation (HPMA-HS-RGD) and three other hydrogels polymerized in the presence of a solid porogen: HPMA-SP, HPMA-SP-RGD, and hydroxy ethyl methacrylate [2-(methacryloyloxy)ethyl] trimethylammonium chloride (HEMA-MOETACl). Their adhesion capability and cell survival were evaluated at 1, 7, and 14 days after the seeding of MSCs on the hydrogel scaffolds. The cell-polymer scaffolds were then implanted into hemisected rat spinal cord, and MSC survival in vivo and the ingrowth of endogenous tissue elements were evaluated 1 month after implantation. In vitro data demonstrated that HEMA-MOETACl and HPMA-SP-RGD hydrogels were superior in the number of cells attached. In vivo, the highest cell survival was found in the HEMA-MOETACl hydrogels; however, only a small ingrowth of blood vessels and axons was observed. Both HPMA-SP and HPMA-SP-RGD hydrogels showed better survival of MSCs compared with the HPMA-HS-RGD hydrogel.
dcterms:title
Adjusting the Chemical and Physical Properties of Hydrogels Leads to Improved Stem Cell Survival and Tissue Ingrowth in Spinal Cord Injury Reconstruction: A Comparative Study of Four Methacrylate Hydrogels Adjusting the Chemical and Physical Properties of Hydrogels Leads to Improved Stem Cell Survival and Tissue Ingrowth in Spinal Cord Injury Reconstruction: A Comparative Study of Four Methacrylate Hydrogels
skos:prefLabel
Adjusting the Chemical and Physical Properties of Hydrogels Leads to Improved Stem Cell Survival and Tissue Ingrowth in Spinal Cord Injury Reconstruction: A Comparative Study of Four Methacrylate Hydrogels Adjusting the Chemical and Physical Properties of Hydrogels Leads to Improved Stem Cell Survival and Tissue Ingrowth in Spinal Cord Injury Reconstruction: A Comparative Study of Four Methacrylate Hydrogels
skos:notation
RIV/00216208:11130/13:10209662!RIV14-MSM-11130___
n17:predkladatel
n18:orjk%3A11130
n3:aktivita
n12:S n12:P n12:I
n3:aktivity
I, P(GAP108/10/1560), P(GPP304/11/P633), P(IAA500390902), S
n3:cisloPeriodika
20
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
n4:2490420 n4:4798201 n4:9271562 n4:6738109
n3:druhVysledku
n13:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
59566
n3:idVysledku
RIV/00216208:11130/13:10209662
n3:jazykVysledku
n6:eng
n3:klicovaSlova
hematopoietic-cells; axonal regeneration; intrathecal delivery; polyelectrolyte complexes; 2-hydroxyethyl methacrylate; functional recovery; chronic paraplegic rats; negative surface-charges; colony-stimulating factor; marrow stromal cells
n3:klicoveSlovo
n8:colony-stimulating%20factor n8:hematopoietic-cells n8:functional%20recovery n8:polyelectrolyte%20complexes n8:intrathecal%20delivery n8:2-hydroxyethyl%20methacrylate n8:marrow%20stromal%20cells n8:axonal%20regeneration n8:negative%20surface-charges n8:chronic%20paraplegic%20rats
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[60BAA040B4BF]
n3:nazevZdroje
Stem Cells and Development
n3:obor
n20:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
10
n3:projekt
n10:GAP108%2F10%2F1560 n10:IAA500390902 n10:GPP304%2F11%2FP633
n3:rokUplatneniVysledku
n16:2013
n3:svazekPeriodika
22
n3:tvurceVysledku
Pradny, Martin Syková, Eva Jendelová, Pavla Krumbholcova, Eva Michalek, Jiří Turnovcová, Karolína Hejcl, Ales Růžička, Jiří Kapcalova, Miroslava Cihlar, Jiří
n3:wos
000325148800010
s:issn
1547-3287
s:numberOfPages
12
n21:doi
10.1089/scd.2012.0616
n11:organizacniJednotka
11130