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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F13%3A10209593%21RIV14-GA0-11130___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1182/blood-2012-11-468702
dcterms:description
A better description of the leukemia cell surface proteome (surfaceome) is a prerequisite for the development of diagnostic and therapeutic tools. Insights into the complexity of the surfaceome have been limited by the lack of suitable methodologies. We combined a leukemia xenograft model with the discovery-driven chemoproteomic Cell Surface Capture technology to explore the B-cell precursor acute lymphoblastic leukemia (BCP-ALL) surfaceome; 713 cell surface proteins, including 181 CD proteins, were detected through combined analysis of 19 BCP-ALL cases. Diagnostic immunophenotypes were recapitulated in each case, and subtype specific markers were detected. To identify new leukemia-associated markers, we filtered the surfaceome data set against gene expression information from sorted, normal hematopoietic cells. Nine candidate markers (CD18, CD63, CD31, CD97, CD102, CD157, CD217, CD305, and CD317) were validated by flow cytometry in patient samples at diagnosis and during chemotherapy. CD97, CD157, CD63, and CD305 accounted for the most informative differences between normal and malignant cells. The ALL surfaceome constitutes a valuable resource to assist the functional exploration of surface markers in normal and malignant lymphopoiesis. This unbiased approach will also contribute to the development of strategies that rely on complex information for multidimensional flow cytometry data analysis to improve its diagnostic applications. A better description of the leukemia cell surface proteome (surfaceome) is a prerequisite for the development of diagnostic and therapeutic tools. Insights into the complexity of the surfaceome have been limited by the lack of suitable methodologies. We combined a leukemia xenograft model with the discovery-driven chemoproteomic Cell Surface Capture technology to explore the B-cell precursor acute lymphoblastic leukemia (BCP-ALL) surfaceome; 713 cell surface proteins, including 181 CD proteins, were detected through combined analysis of 19 BCP-ALL cases. Diagnostic immunophenotypes were recapitulated in each case, and subtype specific markers were detected. To identify new leukemia-associated markers, we filtered the surfaceome data set against gene expression information from sorted, normal hematopoietic cells. Nine candidate markers (CD18, CD63, CD31, CD97, CD102, CD157, CD217, CD305, and CD317) were validated by flow cytometry in patient samples at diagnosis and during chemotherapy. CD97, CD157, CD63, and CD305 accounted for the most informative differences between normal and malignant cells. The ALL surfaceome constitutes a valuable resource to assist the functional exploration of surface markers in normal and malignant lymphopoiesis. This unbiased approach will also contribute to the development of strategies that rely on complex information for multidimensional flow cytometry data analysis to improve its diagnostic applications.
dcterms:title
Leukemia surfaceome analysis reveals new disease-associated features Leukemia surfaceome analysis reveals new disease-associated features
skos:prefLabel
Leukemia surfaceome analysis reveals new disease-associated features Leukemia surfaceome analysis reveals new disease-associated features
skos:notation
RIV/00216208:11130/13:10209593!RIV14-GA0-11130___
n20:predkladatel
n21:orjk%3A11130
n7:aktivita
n15:S n15:P n15:I
n7:aktivity
I, P(GAP301/10/1877), P(NT13462), S
n7:cisloPeriodika
25
n7:dodaniDat
n8:2014
n7:domaciTvurceVysledku
n9:2780909 n9:8861374
n7:druhVysledku
n17:J
n7:duvernostUdaju
n12:S
n7:entitaPredkladatele
n11:predkladatel
n7:idSjednocenehoVysledku
84785
n7:idVysledku
RIV/00216208:11130/13:10209593
n7:jazykVysledku
n18:eng
n7:klicovaSlova
childhood; aieop-bfm; cell-lines; mass cytometry; prognostic-factors; lymphocytic-leukemia; bone-marrow; childrens oncology group; minimal residual disease; acute lymphoblastic-leukemia
n7:klicoveSlovo
n10:mass%20cytometry n10:aieop-bfm n10:bone-marrow n10:acute%20lymphoblastic-leukemia n10:minimal%20residual%20disease n10:childhood n10:cell-lines n10:lymphocytic-leukemia n10:childrens%20oncology%20group n10:prognostic-factors
n7:kodStatuVydavatele
US - Spojené státy americké
n7:kontrolniKodProRIV
[AF9C9DBDA1CB]
n7:nazevZdroje
Blood
n7:obor
n19:FD
n7:pocetDomacichTvurcuVysledku
2
n7:pocetTvurcuVysledku
14
n7:projekt
n13:NT13462 n13:GAP301%2F10%2F1877
n7:rokUplatneniVysledku
n8:2013
n7:svazekPeriodika
121
n7:tvurceVysledku
Mirkowska, Paulina Mejstříková, Ester Bourquin, Jean-Pierre Cario, Gunnar van der Velden, Vincent H. J. Schmitz, Maike Schrappe, Martin Hofmann, Andreas Slámová, Lucie Sedek, Lukasz Szczepanski, Tomasz Bornhauser, Beat C. Stanulla, Martin Wollscheid, Bernd
n7:wos
000321898500001
s:issn
0006-4971
s:numberOfPages
11
n16:doi
10.1182/blood-2012-11-468702
n6:organizacniJednotka
11130