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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F13%3A10135339%21RIV14-GA0-11130___
rdf:type
skos:Concept n10:Vysledek
rdfs:seeAlso
http://www.electrochemsci.org/papers/vol8/80404472.pdf
dcterms:description
Platinum-based cytostatics play an important role in the chemotherapy of various tumour diseases. Therefore, it is not surprising that there is a great effort for studying of the cytostatics and their fate in an organism. In this study, we aimed our attention at determination of cisplatin, carboplatin and oxaliplatin using stationary and flow electrochemical methods. Primarily, determination of the platinum based cytostatics using differential pulse voltammetry at hanging mercury drop electrode was optimized. Under the optimal conditions (supporting electrolyte: 2 ml of 0.36 M sulphuric acid containing 0.24 ml of hydrazine (10 mM) and 0.01 ml of formaldehyde (37 % aqueous solution, v/v), pH of the supporting electrolyte: 1.8, potential of accumulation: -0.7 V, time of accumulation: 120 s), limits of detection were estimated (3 S/N) down to tens of pg per ml for the studied drugs. Further, we investigated the interactions of the characterized drugs with peptide fragments of protein metallothionein, because the overexpression of metallothionein in tumour cells belongs to the one of the generally accepted mechanisms of resistance to these cytostatics. For this purpose, flow injection analysis with electrochemical detection was utilized. As it is well evident from the obtained experimental data, interactions between peptide fragments and platinum-based complexes proceeded differently, where oxaliplatin demonstrated the highest ability to form complex. Platinum-based cytostatics play an important role in the chemotherapy of various tumour diseases. Therefore, it is not surprising that there is a great effort for studying of the cytostatics and their fate in an organism. In this study, we aimed our attention at determination of cisplatin, carboplatin and oxaliplatin using stationary and flow electrochemical methods. Primarily, determination of the platinum based cytostatics using differential pulse voltammetry at hanging mercury drop electrode was optimized. Under the optimal conditions (supporting electrolyte: 2 ml of 0.36 M sulphuric acid containing 0.24 ml of hydrazine (10 mM) and 0.01 ml of formaldehyde (37 % aqueous solution, v/v), pH of the supporting electrolyte: 1.8, potential of accumulation: -0.7 V, time of accumulation: 120 s), limits of detection were estimated (3 S/N) down to tens of pg per ml for the studied drugs. Further, we investigated the interactions of the characterized drugs with peptide fragments of protein metallothionein, because the overexpression of metallothionein in tumour cells belongs to the one of the generally accepted mechanisms of resistance to these cytostatics. For this purpose, flow injection analysis with electrochemical detection was utilized. As it is well evident from the obtained experimental data, interactions between peptide fragments and platinum-based complexes proceeded differently, where oxaliplatin demonstrated the highest ability to form complex.
dcterms:title
Interactions of Platinum-Based Cytostatics with Metallothionein Revealed by Electrochemistry Interactions of Platinum-Based Cytostatics with Metallothionein Revealed by Electrochemistry
skos:prefLabel
Interactions of Platinum-Based Cytostatics with Metallothionein Revealed by Electrochemistry Interactions of Platinum-Based Cytostatics with Metallothionein Revealed by Electrochemistry
skos:notation
RIV/00216208:11130/13:10135339!RIV14-GA0-11130___
n10:predkladatel
n11:orjk%3A11130
n4:aktivita
n5:P n5:I
n4:aktivity
I, P(ED1.1.00/02.0068), P(EE2.4.31.0023), P(GAP301/10/0356)
n4:cisloPeriodika
4
n4:dodaniDat
n8:2014
n4:domaciTvurceVysledku
n19:9974687
n4:druhVysledku
n17:J
n4:duvernostUdaju
n9:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
80668
n4:idVysledku
RIV/00216208:11130/13:10135339
n4:jazykVysledku
n13:eng
n4:klicovaSlova
anticancer therapy; flow injection analysis; voltammetry; metallomics; metallothionein; oxaliplatin; carboplatin; cisplatin
n4:klicoveSlovo
n6:metallomics n6:anticancer%20therapy n6:metallothionein n6:flow%20injection%20analysis n6:voltammetry n6:carboplatin n6:cisplatin n6:oxaliplatin
n4:kodStatuVydavatele
RS - Srbská republika
n4:kontrolniKodProRIV
[999B78FDBFF1]
n4:nazevZdroje
International Journal of Electrochemical Science
n4:obor
n15:CG
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
12
n4:projekt
n12:GAP301%2F10%2F0356 n12:ED1.1.00%2F02.0068 n12:EE2.4.31.0023
n4:rokUplatneniVysledku
n8:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Stiborová, Marie Zítka, Ondřej Adam, Vojtěch Kenšová, Renáta Kizek, René Eckschlager, Tomáš Beklová, Miroslava Šmerková, Kristýna Hubálek, Jaromír Kremplová, Monika Hynek, David Trnková, Libuše
n4:wos
000318217200009
s:issn
1452-3981
s:numberOfPages
13
n18:organizacniJednotka
11130