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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F11%3A7266%21RIV12-MZ0-11130___
rdf:type
skos:Concept n13:Vysledek
rdfs:seeAlso
http://www.translational-medicine.com/content/pdf/1479-5876-9-223.pdf
dcterms:description
Background: For clinical applications, dendritic cells (DCs) need to be generated using GMP-approved reagents. In this study, we tested the characteristics of DCs generated in two clinical grade culture media and activated by three maturation stimuli, Poly I: C, LPS and the mixture of proinflammatory cytokines in order to identify the optimal combination of culture media and activation stimulus for the clinical use. Method: We tested DCs generation using two GMP-certified culture media, CellGro and RPMI+5% human AB serum and evaluated DCs morphology, viability and capapability to mature. We tested three maturation stimuli, PolyI:C, LPS and the mixture of proinflammatory cytokines consisting of IL-1, IL-6, TNF and prostaglandin E2. We evaluated the capacity of activated DCs to induce antigen-specific T cells and regulatory T lymphocytes. Results: Cell culture in CellGro resulted in a higher yield of immature DCs resulting from increased number of adherent monocytes. DCs that were generated in CellGro and activated using Poly I:C were the most efficient in expanding antigen-specific T cells compared to the DCs that were generated in other media and activated using LPS or the cocktail of proinflammatory cytokines. A comparison of all tested combinations revealed that DCs that were generated in CellGro and activated using Poly I:C induced low numbers of regulatory T cells. Conclusion: In this study, we identified monocyte-derived DCs that were generated in CellGro and activated using Poly I:C as the most potent clinical-grade DCs for the induction of antigen-specific T cells. Background: For clinical applications, dendritic cells (DCs) need to be generated using GMP-approved reagents. In this study, we tested the characteristics of DCs generated in two clinical grade culture media and activated by three maturation stimuli, Poly I: C, LPS and the mixture of proinflammatory cytokines in order to identify the optimal combination of culture media and activation stimulus for the clinical use. Method: We tested DCs generation using two GMP-certified culture media, CellGro and RPMI+5% human AB serum and evaluated DCs morphology, viability and capapability to mature. We tested three maturation stimuli, PolyI:C, LPS and the mixture of proinflammatory cytokines consisting of IL-1, IL-6, TNF and prostaglandin E2. We evaluated the capacity of activated DCs to induce antigen-specific T cells and regulatory T lymphocytes. Results: Cell culture in CellGro resulted in a higher yield of immature DCs resulting from increased number of adherent monocytes. DCs that were generated in CellGro and activated using Poly I:C were the most efficient in expanding antigen-specific T cells compared to the DCs that were generated in other media and activated using LPS or the cocktail of proinflammatory cytokines. A comparison of all tested combinations revealed that DCs that were generated in CellGro and activated using Poly I:C induced low numbers of regulatory T cells. Conclusion: In this study, we identified monocyte-derived DCs that were generated in CellGro and activated using Poly I:C as the most potent clinical-grade DCs for the induction of antigen-specific T cells.
dcterms:title
Poly I: C-activated dendritic cells that were generated in CellGro for use in cancer immunotherapy trials Poly I: C-activated dendritic cells that were generated in CellGro for use in cancer immunotherapy trials
skos:prefLabel
Poly I: C-activated dendritic cells that were generated in CellGro for use in cancer immunotherapy trials Poly I: C-activated dendritic cells that were generated in CellGro for use in cancer immunotherapy trials
skos:notation
RIV/00216208:11130/11:7266!RIV12-MZ0-11130___
n13:predkladatel
n14:orjk%3A11130
n3:aktivita
n5:P
n3:aktivity
P(NT11559)
n3:cisloPeriodika
223
n3:dodaniDat
n12:2012
n3:domaciTvurceVysledku
n4:1287362 n4:6291171 n4:8086478 n4:1110047 n4:7629419 n4:5730244 n4:5853826
n3:druhVysledku
n19:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
221187
n3:idVysledku
RIV/00216208:11130/11:7266
n3:jazykVysledku
n8:eng
n3:klicovaSlova
cancer immunotherapy; dendritic cells; Poly I:C; culture media; clinical use
n3:klicoveSlovo
n10:Poly%20I%3AC n10:culture%20media n10:dendritic%20cells n10:cancer%20immunotherapy n10:clinical%20use
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[25610A9B8B8C]
n3:nazevZdroje
Journal of Translational Medicine
n3:obor
n17:EC
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
8
n3:projekt
n20:NT11559
n3:rokUplatneniVysledku
n12:2011
n3:svazekPeriodika
9
n3:tvurceVysledku
Pokorna, K. Ulčová, Hana Fučíková, Jitka Budinský, Vít Rožková, Daniela Sochorová, Klára Bartůňková, Jiřina Špíšek, Radek
n3:wos
000299108200001
s:issn
1479-5876
s:numberOfPages
10
n11:organizacniJednotka
11130