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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F11%3A7027%21RIV12-MZ0-11130___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pubmed/21057553
dcterms:description
Depletion of cellular immunity as a consequence of conditioning before allogeneic hematopoietic SCT (HSCT) frequently results in CMV reactivation, which may in turn lead to life-threatening infections and require timely antiviral treatment. We have investigated the functional signatures of CMV-specific CD4+ and CD8+ T-cells in 191 samples from 118 individuals. We compared healthy donors with both patients with high and undetectable viral loads, and those who controlled and did not control their CMV reactivations. Polychromatic flow-cytometric measurements of CD154 (CD40L), intracellular cytokines (IFN gamma, IL2) and a degranulation marker (CD107a) allowed us to assess the functional status of various T-cells simultaneously. We found that dual IFN gamma/IL2-producing CD8+ T-cells were present in patients controlling their CMV reactivations but absent from non-controllers. CD8+ T-cells that produce only IFN gamma were the most abundant subtype, but they most likely represent non-protective memory cells. Distinct functional signatures were examined by hierarchical clustering, and this revealed that, unlike polyfunctional CD8+ T-cells, CD8+ T-cells that produce IFN gamma alone were not functioning in concert with other subsets. In conclusion, our study revealed functional signatures that may be useful for immune monitoring, and it could change the interpretation of previous studies that assessed only IFN gamma. Bone Marrow Transplantation (2011) 46, 1089-1098; doi: 10.1038/bmt.2010.261; published online 8 November 2010 Depletion of cellular immunity as a consequence of conditioning before allogeneic hematopoietic SCT (HSCT) frequently results in CMV reactivation, which may in turn lead to life-threatening infections and require timely antiviral treatment. We have investigated the functional signatures of CMV-specific CD4+ and CD8+ T-cells in 191 samples from 118 individuals. We compared healthy donors with both patients with high and undetectable viral loads, and those who controlled and did not control their CMV reactivations. Polychromatic flow-cytometric measurements of CD154 (CD40L), intracellular cytokines (IFN gamma, IL2) and a degranulation marker (CD107a) allowed us to assess the functional status of various T-cells simultaneously. We found that dual IFN gamma/IL2-producing CD8+ T-cells were present in patients controlling their CMV reactivations but absent from non-controllers. CD8+ T-cells that produce only IFN gamma were the most abundant subtype, but they most likely represent non-protective memory cells. Distinct functional signatures were examined by hierarchical clustering, and this revealed that, unlike polyfunctional CD8+ T-cells, CD8+ T-cells that produce IFN gamma alone were not functioning in concert with other subsets. In conclusion, our study revealed functional signatures that may be useful for immune monitoring, and it could change the interpretation of previous studies that assessed only IFN gamma. Bone Marrow Transplantation (2011) 46, 1089-1098; doi: 10.1038/bmt.2010.261; published online 8 November 2010
dcterms:title
Signature profiles of CMV-specific T-cells in patients with CMV reactivation after hematopoietic SCT Signature profiles of CMV-specific T-cells in patients with CMV reactivation after hematopoietic SCT
skos:prefLabel
Signature profiles of CMV-specific T-cells in patients with CMV reactivation after hematopoietic SCT Signature profiles of CMV-specific T-cells in patients with CMV reactivation after hematopoietic SCT
skos:notation
RIV/00216208:11130/11:7027!RIV12-MZ0-11130___
n17:predkladatel
n18:orjk%3A11130
n4:aktivita
n12:P n12:Z
n4:aktivity
P(NS9996), Z(MSM0021620813), Z(MZ0FNM2005)
n4:cisloPeriodika
8
n4:dodaniDat
n10:2012
n4:domaciTvurceVysledku
n5:8646597 n5:9336524 n5:2296705 n5:4604334 n5:2842858 n5:1891871 n5:4977939
n4:druhVysledku
n8:J
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
229211
n4:idVysledku
RIV/00216208:11130/11:7027
n4:jazykVysledku
n16:eng
n4:klicovaSlova
human T-cells; hematopoietic SCT; children; CMV; immunologic monitoring; IFN-gamma; IL-2; allogeneic marrow transplantation; human cytomegalovirus-infection; immune reconstitution; functional signatures; viral-infection; cd8+t cells; recipients; cd4(+); cd8(+); disease
n4:klicoveSlovo
n9:functional%20signatures n9:recipients n9:hematopoietic%20SCT n9:CMV n9:cd4%28%2B%29 n9:cd8%28%2B%29 n9:cd8%2Bt%20cells n9:children n9:IL-2 n9:immune%20reconstitution n9:disease n9:allogeneic%20marrow%20transplantation n9:immunologic%20monitoring n9:human%20T-cells n9:human%20cytomegalovirus-infection n9:IFN-gamma n9:viral-infection
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[57D8A6D86208]
n4:nazevZdroje
Bone Marrow Transplantation
n4:obor
n15:FD
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
8
n4:projekt
n13:NS9996
n4:rokUplatneniVysledku
n10:2011
n4:svazekPeriodika
46
n4:tvurceVysledku
Sedláček, Petr Hubáček, Petr Starý, Jan Keslová, Petra Stuchlý, Jan Król, Ladislav Hrušák, Ondřej Kalina, Tomáš
n4:wos
000293779000009
n4:zamer
n21:MZ0FNM2005 n21:MSM0021620813
s:issn
0268-3369
s:numberOfPages
10
n7:organizacniJednotka
11130