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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F14%3A43909166%21RIV15-MSM-11120___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
INTRODUCTION: Results of clinical trials have demonstrated that circulating tumour cells (CTCs) are frequently detected in patients with urothelial tumours. The monitoring of CTCs has the potential to improve therapeutic management at an early stage and also to identify patients with increased risk of tumour progression or recurrence before the onset of clinically detected metastasis. In this study, we report a new effectively simplified methodology for a separation and in vitro culturing of viable CTCs from peripheral blood. METHOD: We include patients diagnosed with 3 types of urothelial tumours (prostate cancer, urinary bladder cancer, and kidney cancer). A size-based separation method for viable CTC - enrichment from unclothed peripheral blood has been introduced (MetaCell, Ostrava, Czech Republic). The enriched CTCs fraction was cultured directly on the separation membrane, or transferred from the membrane and cultured on any plastic surface or a microscopic slide. RESULTS: We report a successful application of a CTCs isolation procedure in patients with urothelial cancers. The CTCs captured on the membrane are enriched with a remarkable proliferation potential. This has enabled us to set up in vitro cell cultures from the viable CTCs unaffected by any fixation buffers, antibodies or lysing solutions. Next, the CTCs were cultured in vitro for a minimum of 10 to 14 days to enable further downstream analysis (e.g., immunohistochemistry). CONCLUSION: We demonstrated an efficient CTCs capture platform, based on a cell size separation principle. Furthermore, we report an ability to culture the enriched cells - a critical requirement for post-isolation cellular analysis. INTRODUCTION: Results of clinical trials have demonstrated that circulating tumour cells (CTCs) are frequently detected in patients with urothelial tumours. The monitoring of CTCs has the potential to improve therapeutic management at an early stage and also to identify patients with increased risk of tumour progression or recurrence before the onset of clinically detected metastasis. In this study, we report a new effectively simplified methodology for a separation and in vitro culturing of viable CTCs from peripheral blood. METHOD: We include patients diagnosed with 3 types of urothelial tumours (prostate cancer, urinary bladder cancer, and kidney cancer). A size-based separation method for viable CTC - enrichment from unclothed peripheral blood has been introduced (MetaCell, Ostrava, Czech Republic). The enriched CTCs fraction was cultured directly on the separation membrane, or transferred from the membrane and cultured on any plastic surface or a microscopic slide. RESULTS: We report a successful application of a CTCs isolation procedure in patients with urothelial cancers. The CTCs captured on the membrane are enriched with a remarkable proliferation potential. This has enabled us to set up in vitro cell cultures from the viable CTCs unaffected by any fixation buffers, antibodies or lysing solutions. Next, the CTCs were cultured in vitro for a minimum of 10 to 14 days to enable further downstream analysis (e.g., immunohistochemistry). CONCLUSION: We demonstrated an efficient CTCs capture platform, based on a cell size separation principle. Furthermore, we report an ability to culture the enriched cells - a critical requirement for post-isolation cellular analysis.
dcterms:title
Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture
skos:prefLabel
Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture Circulating tumour cells in patients with urothelial tumours: Enrichment and in vitro culture
skos:notation
RIV/00216208:11120/14:43909166!RIV15-MSM-11120___
n3:aktivita
n15:S
n3:aktivity
S
n3:cisloPeriodika
9-10
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n4:9313605 n4:1993526 n4:5704995
n3:druhVysledku
n16:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
7326
n3:idVysledku
RIV/00216208:11120/14:43909166
n3:jazykVysledku
n7:eng
n3:klicovaSlova
cultivation; MetaCell; circulating tumor cells; kidney cancer; prostate cancer; Urinary bladder cancer
n3:klicoveSlovo
n6:prostate%20cancer n6:cultivation n6:MetaCell n6:Urinary%20bladder%20cancer n6:kidney%20cancer n6:circulating%20tumor%20cells
n3:kodStatuVydavatele
CA - Kanada
n3:kontrolniKodProRIV
[558D22927B72]
n3:nazevZdroje
Canadian Urological Association Journal
n3:obor
n8:FD
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
8
n3:tvurceVysledku
Kološtová, Katarína Bobek, Vladimír Čegan, Martin
n3:wos
000347710000008
s:issn
1911-6470
s:numberOfPages
6
n14:doi
10.5489/cuaj.1978
n5:organizacniJednotka
11120