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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F14%3A43908867%21RIV15-AV0-11120___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. Nevertheless, 50-100 nm large aggregates formed from the RPMI medium without FBS facilitated cell adhesion also on hydrophobic hydrogenated NCD (increase from 23% to 61%). The authors discuss applicability for biomedical uses. The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. Nevertheless, 50-100 nm large aggregates formed from the RPMI medium without FBS facilitated cell adhesion also on hydrophobic hydrogenated NCD (increase from 23% to 61%). The authors discuss applicability for biomedical uses.
dcterms:title
Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes
skos:prefLabel
Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes Epithelial cell morphology and adhesion on diamond films deposited and chemically modified by plasma processes
skos:notation
RIV/00216208:11120/14:43908867!RIV15-AV0-11120___
n3:aktivita
n19:P
n3:aktivity
P(KAN200100801)
n3:cisloPeriodika
3
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n4:7449186
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
14870
n3:idVysledku
RIV/00216208:11120/14:43908867
n3:jazykVysledku
n7:eng
n3:klicovaSlova
dna; spectroscopy; terminated diamond; nanocrystalline diamond; atomic-force microscopy
n3:klicoveSlovo
n12:dna n12:nanocrystalline%20diamond n12:atomic-force%20microscopy n12:spectroscopy n12:terminated%20diamond
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[BCDE2A2D0DEB]
n3:nazevZdroje
Biointerphases
n3:obor
n17:JI
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n10:KAN200100801
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
9
n3:tvurceVysledku
Mandys, Václav
n3:wos
000340893400013
s:issn
1934-8630
s:numberOfPages
8
n5:doi
10.1116/1.4890471
n11:organizacniJednotka
11120