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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F14%3A43908818%21RIV15-MSM-11120___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
In the present study, we demonstrate an animal model and recently introduced size-based exclusion method for circulating tumor cells (CTCs) isolation. The methodology enables subsequent in vitro CTC-culture and characterization. Human lung cancer cell line H460, expressing red fluorescent protein (H460-RFP), was orthotopically implanted in nude mice. CTCs were isolated by a size-based filtration method and successfully cultured in vitro on the separating membrane (MetaCell®), analyzed by means of time-lapse imaging. The cultured CTCs were heterogeneous in size and morphology even though they originated from a single tumor. The outer CTC-membranes were blebbing in general. Abnormal mitosis resulting in three daughter cells was frequently observed. The expression of RFP ensured that the CTCs originated from lung tumor. These readily isolatable, identifiable and cultivable CTCs can be used to characterize individual patient cancers and for screening of more effective treatment. In the present study, we demonstrate an animal model and recently introduced size-based exclusion method for circulating tumor cells (CTCs) isolation. The methodology enables subsequent in vitro CTC-culture and characterization. Human lung cancer cell line H460, expressing red fluorescent protein (H460-RFP), was orthotopically implanted in nude mice. CTCs were isolated by a size-based filtration method and successfully cultured in vitro on the separating membrane (MetaCell®), analyzed by means of time-lapse imaging. The cultured CTCs were heterogeneous in size and morphology even though they originated from a single tumor. The outer CTC-membranes were blebbing in general. Abnormal mitosis resulting in three daughter cells was frequently observed. The expression of RFP ensured that the CTCs originated from lung tumor. These readily isolatable, identifiable and cultivable CTCs can be used to characterize individual patient cancers and for screening of more effective treatment.
dcterms:title
In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein
skos:prefLabel
In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein
skos:notation
RIV/00216208:11120/14:43908818!RIV15-MSM-11120___
n3:aktivita
n17:I
n3:aktivity
I
n3:cisloPeriodika
5
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n6:5704995 n6:9313605
n3:druhVysledku
n5:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
21072
n3:idVysledku
RIV/00216208:11120/14:43908818
n3:jazykVysledku
n4:eng
n3:klicovaSlova
RFP; Fluorescence; Circulating tumor cells; Orthotopic; Lung cancer
n3:klicoveSlovo
n10:Circulating%20tumor%20cells n10:Fluorescence n10:RFP n10:Orthotopic n10:Lung%20cancer
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[63B0695E2817]
n3:nazevZdroje
Journal of Fluorescence
n3:obor
n16:FD
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
24
n3:tvurceVysledku
Kološtová, Katarína Bobek, Vladimír
n3:wos
000342064100020
s:issn
1053-0509
s:numberOfPages
6
n13:doi
10.1007/s10895-014-1439-3
n14:organizacniJednotka
11120