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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F14%3A43908436%21RIV15-MSM-11120___
rdf:type
n16:Vysledek skos:Concept
dcterms:description
Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14 cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35 s and the LOD is 0.3 µM. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 µM and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1 % for urine and 0.7 % for serum; RSD for the peak areas equalled 1.4 % for urine and 2.6 % for serum. The developed CE technique was tested on performance of routine analyses of metformin in the urine and serum of patients suffering from type II diabetes mellitus. Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14 cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35 s and the LOD is 0.3 µM. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 µM and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1 % for urine and 0.7 % for serum; RSD for the peak areas equalled 1.4 % for urine and 2.6 % for serum. The developed CE technique was tested on performance of routine analyses of metformin in the urine and serum of patients suffering from type II diabetes mellitus.
dcterms:title
Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
skos:prefLabel
Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
skos:notation
RIV/00216208:11120/14:43908436!RIV15-MSM-11120___
n3:aktivita
n4:I
n3:aktivity
I
n3:cisloPeriodika
June 6
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n13:5923492
n3:druhVysledku
n6:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
25673
n3:idVysledku
RIV/00216208:11120/14:43908436
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Rapid separation; Large volume sample stacking; Contactless conductivity detection; Capillary electrophoresis; Antidiabetic drug
n3:klicoveSlovo
n7:Contactless%20conductivity%20detection n7:Rapid%20separation n7:Large%20volume%20sample%20stacking n7:Antidiabetic%20drug n7:Capillary%20electrophoresis
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[30BA5CF5B96F]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
1345
n3:tvurceVysledku
Tůma, Petr
n3:wos
000336709300023
s:issn
0021-9673
s:numberOfPages
5
n14:doi
10.1016/j.chroma.2014.04.016
n10:organizacniJednotka
11120