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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F13%3A43907513%21RIV14-MSM-11120___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
It has been shown in previous studies that liver HEP-G2 cells (human hepatocellular carcinoma) lose their ability to express active alcohol dehydrogenase (ADH) and cytochrome P450 2E1 (CYP2E1). Although both are ethanol-inducible enzymes, short-term exposure to ethanol does not cause any changes in expression or activity in cultured HEP-G2 cells. Therefore, we tested the effect of long-term exposure to ethanol on the expression and activity of both ADH and CYP2E1 in these cells. The expression of ADH and CYP2E1 was assessed at the mRNA and/or protein level using real-time PCR and Western blot analysis. Specific colorimetric assays were used for the measurement of ADH and CYP2E1 enzymatic activities. Caco-2 cells (active CYP2E1 and inactive ADH) were used as control cells. Significantly increased protein expression of ADH (about 2.5-fold) as well as CYP2E1 (about 1.6-fold) was found in HEP-G2 cells after long-term (12 mo) exposure to ethanol. The activity of ADH and CYP2E1 was also significantly increased from 12 ± 3 and 6 ± 1 nmol/h/mg of total protein to 191 ± 9 and 57 ± 9 nmol/h/mg of total protein, respectively. We suggest that the loss of activity of ethanol-metabolizing enzymes in cultured HEP-G2 cells is reversible and can be induced by prolonged exposure to ethanol. We are therefore able to reactivate HEP-G2 cells metabolic functions concerning ethanol oxidation just by modification of in vitro culture conditions without necessity of transfection with its side effect - enzyme overexpression. It has been shown in previous studies that liver HEP-G2 cells (human hepatocellular carcinoma) lose their ability to express active alcohol dehydrogenase (ADH) and cytochrome P450 2E1 (CYP2E1). Although both are ethanol-inducible enzymes, short-term exposure to ethanol does not cause any changes in expression or activity in cultured HEP-G2 cells. Therefore, we tested the effect of long-term exposure to ethanol on the expression and activity of both ADH and CYP2E1 in these cells. The expression of ADH and CYP2E1 was assessed at the mRNA and/or protein level using real-time PCR and Western blot analysis. Specific colorimetric assays were used for the measurement of ADH and CYP2E1 enzymatic activities. Caco-2 cells (active CYP2E1 and inactive ADH) were used as control cells. Significantly increased protein expression of ADH (about 2.5-fold) as well as CYP2E1 (about 1.6-fold) was found in HEP-G2 cells after long-term (12 mo) exposure to ethanol. The activity of ADH and CYP2E1 was also significantly increased from 12 ± 3 and 6 ± 1 nmol/h/mg of total protein to 191 ± 9 and 57 ± 9 nmol/h/mg of total protein, respectively. We suggest that the loss of activity of ethanol-metabolizing enzymes in cultured HEP-G2 cells is reversible and can be induced by prolonged exposure to ethanol. We are therefore able to reactivate HEP-G2 cells metabolic functions concerning ethanol oxidation just by modification of in vitro culture conditions without necessity of transfection with its side effect - enzyme overexpression.
dcterms:title
Alcohol dehydrogenase and cytochrome P450 2E1 can be induced by long-term exposure to ethanol in cultured liver HEP-G2 cells Alcohol dehydrogenase and cytochrome P450 2E1 can be induced by long-term exposure to ethanol in cultured liver HEP-G2 cells
skos:prefLabel
Alcohol dehydrogenase and cytochrome P450 2E1 can be induced by long-term exposure to ethanol in cultured liver HEP-G2 cells Alcohol dehydrogenase and cytochrome P450 2E1 can be induced by long-term exposure to ethanol in cultured liver HEP-G2 cells
skos:notation
RIV/00216208:11120/13:43907513!RIV14-MSM-11120___
n17:predkladatel
n18:orjk%3A11120
n3:aktivita
n7:I n7:S n7:Z
n3:aktivity
I, S, Z(MSM0021620814)
n3:cisloPeriodika
8
n3:dodaniDat
n15:2014
n3:domaciTvurceVysledku
n6:7090277 n6:1758330
n3:druhVysledku
n13:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
60018
n3:idVysledku
RIV/00216208:11120/13:43907513
n3:jazykVysledku
n4:eng
n3:klicovaSlova
HEP-G2 cells; Ethanol; Cytochrome P450 2E1 (CYP2E1); Alcohol dehydrogenase (ADH)
n3:klicoveSlovo
n8:HEP-G2%20cells n8:Ethanol n8:Cytochrome%20P450%202E1%20%28CYP2E1%29 n8:Alcohol%20dehydrogenase%20%28ADH%29
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7BF3DF191629]
n3:nazevZdroje
In Vitro Cellular & Developmental Biology-Animal
n3:obor
n20:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n15:2013
n3:svazekPeriodika
49
n3:tvurceVysledku
Balušíková, Kamila Kovář, Jan
n3:wos
000324646300007
n3:zamer
n16:MSM0021620814
s:issn
1071-2690
s:numberOfPages
7
n9:doi
10.1007/s11626-013-9636-y
n12:organizacniJednotka
11120