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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F13%3A43907498%21RIV14-MSM-11120___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Alterations in white adipose tissue (WAT) function, including changes in protein (adipokine) secretion and extracellular matrix (ECM) composition, promote an insulin-resistant state. We set out to identify novel adipokines regulated by body fat mass in human subcutaneous WAT with potential roles in adipose function. Adipose transcriptome data and secretome profiles from conditions with increased/decreased WAT mass were combined. WAT donors were predominantly women. In vitro effects were assessed using recombinant protein. Results were confirmed by quantitative PCR/ELISA, metabolic assays and immunochemistry in human WAT and adipocytes. We identified a hitherto uncharacterised adipokine, semaphorin 3C (SEMA3C), the expression of which correlated significantly with body weight, insulin resistance (HOMA of insulin resistance [HOMA(IR)], and the rate constant for the insulin tolerance test [K-ITT]) and adipose tissue morphology (hypertrophy vs hyperplasia). SEMA3C was primarily found in mature adipocytes and had no direct effect on human adipocyte differentiation, lipolysis, glucose transport or the expression of beta-oxidation genes. This could in part be explained by the significant downregulation of its cognate receptors during adipogenesis. In contrast, in pre-adipocytes, SEMA3C increased the production/secretion of several ECM components (fibronectin, elastin and collagen I) and matricellular factors (connective tissue growth factor, IL6 and transforming growth factor-beta 1). Furthermore, the expression of SEMA3C in human WAT correlated positively with the degree of fibrosis in WAT. SEMA3C is a novel adipokine regulated by weight changes. The correlation with WAT hypertrophy and fibrosis in vivo, as well as its effects on ECM production in human pre-adipocytes in vitro, together suggest that SEMA3C constitutes an adipocyte-derived paracrine signal that influences ECM composition and may play a pathophysiological role in human WAT. Alterations in white adipose tissue (WAT) function, including changes in protein (adipokine) secretion and extracellular matrix (ECM) composition, promote an insulin-resistant state. We set out to identify novel adipokines regulated by body fat mass in human subcutaneous WAT with potential roles in adipose function. Adipose transcriptome data and secretome profiles from conditions with increased/decreased WAT mass were combined. WAT donors were predominantly women. In vitro effects were assessed using recombinant protein. Results were confirmed by quantitative PCR/ELISA, metabolic assays and immunochemistry in human WAT and adipocytes. We identified a hitherto uncharacterised adipokine, semaphorin 3C (SEMA3C), the expression of which correlated significantly with body weight, insulin resistance (HOMA of insulin resistance [HOMA(IR)], and the rate constant for the insulin tolerance test [K-ITT]) and adipose tissue morphology (hypertrophy vs hyperplasia). SEMA3C was primarily found in mature adipocytes and had no direct effect on human adipocyte differentiation, lipolysis, glucose transport or the expression of beta-oxidation genes. This could in part be explained by the significant downregulation of its cognate receptors during adipogenesis. In contrast, in pre-adipocytes, SEMA3C increased the production/secretion of several ECM components (fibronectin, elastin and collagen I) and matricellular factors (connective tissue growth factor, IL6 and transforming growth factor-beta 1). Furthermore, the expression of SEMA3C in human WAT correlated positively with the degree of fibrosis in WAT. SEMA3C is a novel adipokine regulated by weight changes. The correlation with WAT hypertrophy and fibrosis in vivo, as well as its effects on ECM production in human pre-adipocytes in vitro, together suggest that SEMA3C constitutes an adipocyte-derived paracrine signal that influences ECM composition and may play a pathophysiological role in human WAT.
dcterms:title
Semaphorin 3C is a novel adipokine linked to extracellular matrix composition Semaphorin 3C is a novel adipokine linked to extracellular matrix composition
skos:prefLabel
Semaphorin 3C is a novel adipokine linked to extracellular matrix composition Semaphorin 3C is a novel adipokine linked to extracellular matrix composition
skos:notation
RIV/00216208:11120/13:43907498!RIV14-MSM-11120___
n6:predkladatel
n18:orjk%3A11120
n3:aktivita
n4:R
n3:aktivity
R
n3:cisloPeriodika
8
n3:dodaniDat
n10:2014
n3:domaciTvurceVysledku
n13:3966577
n3:druhVysledku
n19:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
104599
n3:idVysledku
RIV/00216208:11120/13:43907498
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Type 2 diabetes; Obesity; Metabolism; Insulin sensitivity; Fibrosis; Cancer cachexia; Bariatric surgery
n3:klicoveSlovo
n5:Insulin%20sensitivity n5:Bariatric%20surgery n5:Type%202%20diabetes n5:Metabolism n5:Obesity n5:Cancer%20cachexia n5:Fibrosis
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[F974E48060E7]
n3:nazevZdroje
Diabetologia
n3:obor
n15:FB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
18
n3:rokUplatneniVysledku
n10:2013
n3:svazekPeriodika
56
n3:tvurceVysledku
Štich, Vladimír
n3:wos
000321285400015
s:issn
0012-186X
s:numberOfPages
10
n7:doi
10.1007/s00125-013-2931-z
n17:organizacniJednotka
11120