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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F13%3A43907490%21RIV14-MSM-11120___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
We studied the effects of carbohydrate availability on growth, differentiation and metabolism of C2C12 myoblasts and myotubes.We measured growth rates, ability to differentiate, citrate synthase and respiratory chain activities and several parameters of mitochondrial respiration in C2C12 cells grown in media with varying carbohydrate availability (5 g/l glucose, 1 g/l glucose, 1 g/l galactose, and no added carbohydrates). C2C12 myoblasts grow more slowly without glucose irrespective of the presence of galactose, which is not consumed by the cells, and they fail to differentiate without glucose in the medium. Cells grown in a no-glucose medium (with or without galactose) have lower maximal respiration and spare respiratory capacity than cells grown in the presence of glucose. However, increasing glucose concentration above physiological levels decreases the achievable maximal respiration. C2C12 myotubes differentiated at a high glucose concentration showed higher dependency on oxidative respiration under basal conditions but had lower maximal and spare respiratory capacity when compared to cells differentiated under low glucose condition. Citrate synthase activity or mitochondrial yield were not significantly affected by changes in the available substrate concentration but a trend towards a higher respiratory chain activity was observed at reduced glucose levels.Our results show that using galactose to increase oxidative metabolism may not be applicable to every cell line, and the changes in mitochondrial respiratory parameters associated with treating cells with galactose are mainly due to glucose deprivation. Moderate concentrations of glucose (1 g/l) in a growth medium are optimal for mitochondrial respiration in C2C12 cell line while supraphysiological concentrations of glucose cause mitochondrial dysfunction in C2C12 myoblasts and myotubes. We studied the effects of carbohydrate availability on growth, differentiation and metabolism of C2C12 myoblasts and myotubes.We measured growth rates, ability to differentiate, citrate synthase and respiratory chain activities and several parameters of mitochondrial respiration in C2C12 cells grown in media with varying carbohydrate availability (5 g/l glucose, 1 g/l glucose, 1 g/l galactose, and no added carbohydrates). C2C12 myoblasts grow more slowly without glucose irrespective of the presence of galactose, which is not consumed by the cells, and they fail to differentiate without glucose in the medium. Cells grown in a no-glucose medium (with or without galactose) have lower maximal respiration and spare respiratory capacity than cells grown in the presence of glucose. However, increasing glucose concentration above physiological levels decreases the achievable maximal respiration. C2C12 myotubes differentiated at a high glucose concentration showed higher dependency on oxidative respiration under basal conditions but had lower maximal and spare respiratory capacity when compared to cells differentiated under low glucose condition. Citrate synthase activity or mitochondrial yield were not significantly affected by changes in the available substrate concentration but a trend towards a higher respiratory chain activity was observed at reduced glucose levels.Our results show that using galactose to increase oxidative metabolism may not be applicable to every cell line, and the changes in mitochondrial respiratory parameters associated with treating cells with galactose are mainly due to glucose deprivation. Moderate concentrations of glucose (1 g/l) in a growth medium are optimal for mitochondrial respiration in C2C12 cell line while supraphysiological concentrations of glucose cause mitochondrial dysfunction in C2C12 myoblasts and myotubes.
dcterms:title
Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes
skos:prefLabel
Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes
skos:notation
RIV/00216208:11120/13:43907490!RIV14-MSM-11120___
n11:predkladatel
n12:orjk%3A11120
n3:aktivita
n15:S n15:I
n3:aktivity
I, S
n3:cisloPeriodika
8
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n5:7147503 n5:7496915 Elkalaf, Moustafa
n3:druhVysledku
n17:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
85467
n3:idVysledku
RIV/00216208:11120/13:43907490
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Assay; Chain; Nuclear; Fibroblasts; Differentiation; Cells; Messenger-Rna; Insulin-Resistance; Skeletal-Muscle
n3:klicoveSlovo
n4:Differentiation n4:Assay n4:Fibroblasts n4:Chain n4:Nuclear n4:Skeletal-Muscle n4:Cells n4:Messenger-Rna n4:Insulin-Resistance
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[EBCE91ABBEAE]
n3:nazevZdroje
PLoS One
n3:obor
n8:FB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n7:2013
n3:svazekPeriodika
8
n3:tvurceVysledku
Anděl, Michal Trnka, Jan Elkalaf, Moustafa
n3:wos
000324465000132
s:issn
1932-6203
s:numberOfPages
8
n6:doi
10.1371/journal.pone.0070772
n16:organizacniJednotka
11120