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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F12%3A43902226%21RIV13-MZ0-11120___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
BACKGROUND: Nonobstructive azoospermic (NOA) men can father children after testicular sperm extraction (TESE). They were shown to be at risk of producing chromosomally abnormal spermatozoa, but the number of sperm analysed per patient is usually very low. METHODS: Multicolor fluorescence in situ hybridization was used for detection of chromosome 13, 15, 16, 18, 21, 22, X and Y disomy and diploidy in sperm obtained from NOA men (n=17) and control donors (n=10). At least 500 testicular sperm were scored in each patient to increase the predictability rate of our study. RESULTS: The mean frequency of overall disomy (2.32%) and diploidy (0.80%) found in 13,689 testicular spermatozoa of NOA patients was significantly higher than in the ejaculated sperm of normospermic control donors (0.62% of disomy and 0.29% of diploidy). A highly significant increase in frequencies of chromosome 15, Y and overall disomy (P<0.001), and a significant increase in disomy of chromosome 13 (P=0.002), 16 (P=0.031) and 21 (P=0.018), overall diploidy (P=0.031) and diploidy caused by errors in meiosis I (P=0.011) were observed in the NOA group. CONCLUSIONS: Testicular sperm samples of NOA patients show higher incidence of numerical chromosomal abnormalities compared to ejaculated sperm of control donors. Appropriate genetic counselling is necessary in NOA men undergoing TESE. BACKGROUND: Nonobstructive azoospermic (NOA) men can father children after testicular sperm extraction (TESE). They were shown to be at risk of producing chromosomally abnormal spermatozoa, but the number of sperm analysed per patient is usually very low. METHODS: Multicolor fluorescence in situ hybridization was used for detection of chromosome 13, 15, 16, 18, 21, 22, X and Y disomy and diploidy in sperm obtained from NOA men (n=17) and control donors (n=10). At least 500 testicular sperm were scored in each patient to increase the predictability rate of our study. RESULTS: The mean frequency of overall disomy (2.32%) and diploidy (0.80%) found in 13,689 testicular spermatozoa of NOA patients was significantly higher than in the ejaculated sperm of normospermic control donors (0.62% of disomy and 0.29% of diploidy). A highly significant increase in frequencies of chromosome 15, Y and overall disomy (P<0.001), and a significant increase in disomy of chromosome 13 (P=0.002), 16 (P=0.031) and 21 (P=0.018), overall diploidy (P=0.031) and diploidy caused by errors in meiosis I (P=0.011) were observed in the NOA group. CONCLUSIONS: Testicular sperm samples of NOA patients show higher incidence of numerical chromosomal abnormalities compared to ejaculated sperm of control donors. Appropriate genetic counselling is necessary in NOA men undergoing TESE.
dcterms:title
Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients
skos:prefLabel
Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients Testicular Sperm Aneuploidy in Nonobstructive Azoospermic Patients
skos:notation
RIV/00216208:11120/12:43902226!RIV13-MZ0-11120___
n16:predkladatel
n19:orjk%3A11120
n5:aktivita
n8:S n8:P
n5:aktivity
P(ED1.1.00/02.0068), P(NS9842), P(NS9967), S
n5:cisloPeriodika
7
n5:dodaniDat
n13:2013
n5:domaciTvurceVysledku
n14:3335941 n14:7692641
n5:druhVysledku
n7:J
n5:duvernostUdaju
n12:S
n5:entitaPredkladatele
n11:predkladatel
n5:idSjednocenehoVysledku
174007
n5:idVysledku
RIV/00216208:11120/12:43902226
n5:jazykVysledku
n20:eng
n5:klicovaSlova
FISH; aneuploidy; testicular sperm; nonobstructive azoospermia
n5:klicoveSlovo
n10:FISH n10:testicular%20sperm n10:aneuploidy n10:nonobstructive%20azoospermia
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[D695B1F7D1CE]
n5:nazevZdroje
Human Reproduction
n5:obor
n15:EB
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
4
n5:projekt
n6:ED1.1.00%2F02.0068 n6:NS9842 n6:NS9967
n5:rokUplatneniVysledku
n13:2012
n5:svazekPeriodika
27
n5:tvurceVysledku
Sobotka, Vladimír Heráček, Jiří
n5:wos
000305458800039
s:issn
0268-1161
s:numberOfPages
8
n18:doi
10.1093/humrep/des115
n17:organizacniJednotka
11120