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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F12%3A43901101%21RIV13-MZ0-11120___
rdf:type
n4:Vysledek skos:Concept
dcterms:description
A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7-10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7-10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing
dcterms:title
Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential
skos:prefLabel
Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential
skos:notation
RIV/00216208:11120/12:43901101!RIV13-MZ0-11120___
n4:predkladatel
n5:orjk%3A11120
n6:aktivita
n9:P n9:I
n6:aktivity
I, P(NS10507)
n6:cisloPeriodika
Art. Nr. 727352
n6:dodaniDat
n7:2013
n6:domaciTvurceVysledku
n13:8954429 n13:3442365 n13:7449186 n13:3301184 n13:3407306
n6:druhVysledku
n10:J
n6:duvernostUdaju
n20:S
n6:entitaPredkladatele
n19:predkladatel
n6:idSjednocenehoVysledku
139871
n6:idVysledku
RIV/00216208:11120/12:43901101
n6:jazykVysledku
n14:eng
n6:klicovaSlova
Burns; Cultivation; P63; Pig Dermis; Stem-Cells
n6:klicoveSlovo
n8:P63 n8:Cultivation n8:Pig%20Dermis n8:Stem-Cells n8:Burns
n6:kodStatuVydavatele
US - Spojené státy americké
n6:kontrolniKodProRIV
[256DE36BA73B]
n6:nazevZdroje
The Scientific World Journal
n6:obor
n15:FP
n6:pocetDomacichTvurcuVysledku
5
n6:pocetTvurcuVysledku
6
n6:projekt
n18:NS10507
n6:rokUplatneniVysledku
n7:2012
n6:svazekPeriodika
2012
n6:tvurceVysledku
Mandys, Václav Ševčík, Jan Königová, Radana Zajíček, Robert Matoušková, Eva
n6:wos
000306811600001
s:issn
1537-744X
s:numberOfPages
8
n12:doi
10.1100/2012/727352
n16:organizacniJednotka
11120