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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F12%3A43897446%21RIV13-MZ0-11120___
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1007/s12223-012-0105-x
dcterms:description
Antibodies have different avidities that can be evaluated using modified enzyme-linked immunosorbent assay (ELISA) techniques. We determined levels and avidities of antibodies to light (NFL) and medium (NFM) subunits of neurofilaments and tau protein in serum and cerebrospinal fluid (CSF) from 26 patients and anti-tau antibody levels and their avidities in 20 multiple sclerosis (MS) patients and 20 age- and sex-matched controls. Each sample was analyzed using both standard ELISA and also using a similar ELISA protocol with the addition of urea. The avidities of anti-neurocytoskeletal antibodies were higher in the CSF than those in serum (anti-NFL, p < 0.0001; anti-tau, p < 0.01; anti-NFM, n.s.). There was no relationship between avidities in serum and CSF for individual anti-neurocytoskeletal antibodies. We did not observe the relationship among the avidities of various anti-neurocytoskeletal antibodies. The avidities of anti-tau antibodies in the CSF were significantly higher in the MS patients than those in the controls (p < 0.0001). The study demonstrates the differences in avidities of CSF or serum neurocytoskeletal antibodies measured as the urea resistance by ELISA method. Avidity determination of anti-neurocytoskeletal antibodies could contribute to the evaluation of the immunological status of patients. Antibodies have different avidities that can be evaluated using modified enzyme-linked immunosorbent assay (ELISA) techniques. We determined levels and avidities of antibodies to light (NFL) and medium (NFM) subunits of neurofilaments and tau protein in serum and cerebrospinal fluid (CSF) from 26 patients and anti-tau antibody levels and their avidities in 20 multiple sclerosis (MS) patients and 20 age- and sex-matched controls. Each sample was analyzed using both standard ELISA and also using a similar ELISA protocol with the addition of urea. The avidities of anti-neurocytoskeletal antibodies were higher in the CSF than those in serum (anti-NFL, p < 0.0001; anti-tau, p < 0.01; anti-NFM, n.s.). There was no relationship between avidities in serum and CSF for individual anti-neurocytoskeletal antibodies. We did not observe the relationship among the avidities of various anti-neurocytoskeletal antibodies. The avidities of anti-tau antibodies in the CSF were significantly higher in the MS patients than those in the controls (p < 0.0001). The study demonstrates the differences in avidities of CSF or serum neurocytoskeletal antibodies measured as the urea resistance by ELISA method. Avidity determination of anti-neurocytoskeletal antibodies could contribute to the evaluation of the immunological status of patients.
dcterms:title
Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum
skos:prefLabel
Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum Avidity of anti-neurocytoskeletal antibodies in cerebrospinal fluid and serum
skos:notation
RIV/00216208:11120/12:43897446!RIV13-MZ0-11120___
n10:predkladatel
n12:orjk%3A11120
n3:aktivita
n19:I n19:P
n3:aktivity
I, P(NS10369)
n3:cisloPeriodika
5
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n15:1787977
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
124323
n3:idVysledku
RIV/00216208:11120/12:43897446
n3:jazykVysledku
n13:eng
n3:klicovaSlova
ELISA; multiple sclerosis; serum; cerebrospinal fluid; neurofilament; tau; cytoskleton; avidity; antibodies
n3:klicoveSlovo
n6:multiple%20sclerosis n6:cerebrospinal%20fluid n6:antibodies n6:tau n6:ELISA n6:neurofilament n6:serum n6:cytoskleton n6:avidity
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[48D29F3D0EC2]
n3:nazevZdroje
Folia Microbiologica (Praha)
n3:obor
n20:FH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n11:NS10369
n3:rokUplatneniVysledku
n8:2012
n3:svazekPeriodika
57
n3:tvurceVysledku
Fialová, Lenka Bartoš, Aleš
n3:wos
000307509300004
s:issn
0015-5632
s:numberOfPages
5
n17:doi
10.1007/s12223-012-0105-x
n9:organizacniJednotka
11120