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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F11%3A00002421%21RIV12-GA0-11120___
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.jsb.2010.11.023
dcterms:description
In mammalian cells, transcriptionally active ribosomal genes are replicated in the early S phase, and the silent ribosomal genes in the late S phase, though mechanisms of this timing remain unknown. UBF (Upstream Binding Factor), a DNA binding protein and component of the pol I transcription machinery, is considered to be responsible for the loose chromatin structure of the active rDNA. Here we question whether such structure alone can ensure early replication of DNA. We investigate this problem on the model of pseudo-NORs, the tandem arrays of heterologous DNA sequence with high affinity for UBF, introduced into human chromosomes. Such arrays are not transcribed, yet efficiently bind UBF and mimic the chromatin structure of active rDNA. In our study, a human derived stable cell line containing one pseudo-NOR on the chromosome 10 was transiently transfected with UBF-GFP and PCNA-RFP, which allowed us to observe in vivo the growth of pseudo-NORs resulted from their replication. In mammalian cells, transcriptionally active ribosomal genes are replicated in the early S phase, and the silent ribosomal genes in the late S phase, though mechanisms of this timing remain unknown. UBF (Upstream Binding Factor), a DNA binding protein and component of the pol I transcription machinery, is considered to be responsible for the loose chromatin structure of the active rDNA. Here we question whether such structure alone can ensure early replication of DNA. We investigate this problem on the model of pseudo-NORs, the tandem arrays of heterologous DNA sequence with high affinity for UBF, introduced into human chromosomes. Such arrays are not transcribed, yet efficiently bind UBF and mimic the chromatin structure of active rDNA. In our study, a human derived stable cell line containing one pseudo-NOR on the chromosome 10 was transiently transfected with UBF-GFP and PCNA-RFP, which allowed us to observe in vivo the growth of pseudo-NORs resulted from their replication.
dcterms:title
Replication timing of pseudo-NORs Replication timing of pseudo-NORs
skos:prefLabel
Replication timing of pseudo-NORs Replication timing of pseudo-NORs
skos:notation
RIV/00216208:11120/11:00002421!RIV12-GA0-11120___
n13:predkladatel
n18:orjk%3A11120
n3:aktivita
n19:Z n19:P n19:I
n3:aktivity
I, P(GA304/09/1047), P(LC535), Z(MSM0021620806), Z(MSM0021620858)
n3:cisloPeriodika
2
n3:dodaniDat
n4:2012
n3:domaciTvurceVysledku
n9:7891687
n3:druhVysledku
n22:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
226587
n3:idVysledku
RIV/00216208:11120/11:00002421
n3:jazykVysledku
n6:eng
n3:klicovaSlova
Chromatin structure; Pseudo-NORs; rDNA; Replication; UBF
n3:klicoveSlovo
n7:UBF n7:Replication n7:rDNA n7:Pseudo-NORs n7:Chromatin%20structure
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[1A63EEB7013D]
n3:nazevZdroje
Journal of Structural Biology
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
11
n3:projekt
n17:LC535 n17:GA304%2F09%2F1047
n3:rokUplatneniVysledku
n4:2011
n3:svazekPeriodika
173
n3:tvurceVysledku
Smirnov, Evgeny Raška, Otakar
n3:wos
000286846700003
n3:zamer
n8:MSM0021620858 n8:MSM0021620806
s:issn
1047-8477
s:numberOfPages
6
n5:doi
10.1016/j.jsb.2010.11.023
n21:organizacniJednotka
11120