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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F10%3A00002405%21RIV11-GA0-11120___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
A capillary electrophoretic procedure employing capacitively coupled contactless conductivity detection (C4D) has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min., respectively, when using the optimized background electrolyte, 60 mM H3BO3 + 30 mM LiOH (pH 9.1). The limits of detection amount to 0.5 uM for glycerol and 0.3 uM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 % and 3.2 %, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further A capillary electrophoretic procedure employing capacitively coupled contactless conductivity detection (C4D) has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min., respectively, when using the optimized background electrolyte, 60 mM H3BO3 + 30 mM LiOH (pH 9.1). The limits of detection amount to 0.5 uM for glycerol and 0.3 uM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 % and 3.2 %, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further
dcterms:title
The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance
skos:prefLabel
The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance
skos:notation
RIV/00216208:11120/10:00002405!RIV11-GA0-11120___
n3:aktivita
n11:Z n11:S n11:P
n3:aktivity
P(GA203/07/0896), P(GAP206/10/1231), S, Z(MSM0021620814)
n3:cisloPeriodika
12
n3:dodaniDat
n8:2011
n3:domaciTvurceVysledku
n10:2944146 n10:5923492 n10:6395996 n10:2174812
n3:druhVysledku
n15:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
294641
n3:idVysledku
RIV/00216208:11120/10:00002405
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Adipose tissue; Contactless conductivity detection; Glycerol; Mannitol.
n3:klicoveSlovo
n13:Contactless%20conductivity%20detection n13:Glycerol n13:Mannitol. n13:Adipose%20tissue
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[FD67D3DD2EBD]
n3:nazevZdroje
Electrophoresis
n3:obor
n9:CB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n5:GA203%2F07%2F0896 n5:GAP206%2F10%2F1231
n3:rokUplatneniVysledku
n8:2010
n3:svazekPeriodika
31
n3:tvurceVysledku
Samcová, Eva Wedellová, Zuzana Málková, Klára Tůma, Petr
n3:wos
000280057600013
n3:zamer
n4:MSM0021620814
s:issn
0173-0835
s:numberOfPages
7
n6:organizacniJednotka
11120