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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F09%3A00001676%21RIV10-GA0-11120___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 μM by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol. Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 μM by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
dcterms:title
A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection
skos:prefLabel
A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection
skos:notation
RIV/00216208:11120/09:00001676!RIV10-GA0-11120___
n3:aktivita
n16:P n16:Z
n3:aktivity
P(GA203/07/0896), Z(MSM0021620814), Z(MSM0021620816)
n3:cisloPeriodika
19
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n6:2944146 n6:5916542 n6:5923492
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
301240
n3:idVysledku
RIV/00216208:11120/09:00001676
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Amino acids; Contactless conductivity detection; Neurotransmitters.
n3:klicoveSlovo
n5:Amino%20acids n5:Contactless%20conductivity%20detection n5:Neurotransmitters.
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[37BFDB193E1B]
n3:nazevZdroje
Electrophoresis
n3:obor
n19:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n13:GA203%2F07%2F0896
n3:rokUplatneniVysledku
n8:2009
n3:svazekPeriodika
30
n3:tvurceVysledku
Soukupová, Marie Štulík, Karel Samcová, Eva Tůma, Petr
n3:wos
000271154100019
n3:zamer
n7:MSM0021620814 n7:MSM0021620816
s:issn
0173-0835
s:numberOfPages
6
n14:organizacniJednotka
11120