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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F07%3A00002186%21RIV11-MSM-11120___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
To investigate the site of barrier function to the passive diffusion of a small molecule (phalloidin) in the corneal epithelium in the mouse. Penetration of phalloidin (molecular weight 1115 daltons) into the cornea was evaluated by studying fluorescent binding of phalloidin to actin in tissue sections, in whole mount preparations, and in the fixed intact globe by confocal microscopy. In addition, the location of tight junction proteins in the individual layers of the corneal epithelium was determined by immunohistochemistry. Results: Phalloidin staining of corneal sections was positive in all corneal layers in tissue sections and in all layers of the corneal epithelium except the suprabasal layer in excised fixed whole mounts of the cornea. However, when phalloidin staining was attempted in intact fixed globes, before excision of the cornea for whole mount preparation, only the most superficial layer of cells was stained indicating that phalloidin could not penetrate the tissue beyond the suprabasa To investigate the site of barrier function to the passive diffusion of a small molecule (phalloidin) in the corneal epithelium in the mouse. Penetration of phalloidin (molecular weight 1115 daltons) into the cornea was evaluated by studying fluorescent binding of phalloidin to actin in tissue sections, in whole mount preparations, and in the fixed intact globe by confocal microscopy. In addition, the location of tight junction proteins in the individual layers of the corneal epithelium was determined by immunohistochemistry. Results: Phalloidin staining of corneal sections was positive in all corneal layers in tissue sections and in all layers of the corneal epithelium except the suprabasal layer in excised fixed whole mounts of the cornea. However, when phalloidin staining was attempted in intact fixed globes, before excision of the cornea for whole mount preparation, only the most superficial layer of cells was stained indicating that phalloidin could not penetrate the tissue beyond the suprabasa
dcterms:title
The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes
skos:prefLabel
The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes
skos:notation
RIV/00216208:11120/07:00002186!RIV11-MSM-11120___
n4:aktivita
n15:N
n4:aktivity
N
n4:cisloPeriodika
3
n4:dodaniDat
n12:2011
n4:domaciTvurceVysledku
n5:5108063 n5:4788532
n4:druhVysledku
n13:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
453563
n4:idVysledku
RIV/00216208:11120/07:00002186
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Tight Junction; Protein Expression; Actin-Filaments; Dendritic Cells; Ocular Surface; Zo-1; Population; Membrane; Occludin; Complex
n4:klicoveSlovo
n11:Membrane n11:Occludin n11:Ocular%20Surface n11:Complex n11:Tight%20Junction n11:Dendritic%20Cells n11:Zo-1 n11:Population n11:Actin-Filaments n11:Protein%20Expression
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[F4772D0FBD74]
n4:nazevZdroje
British Journal of Ophthalmology
n4:obor
n8:FF
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n12:2007
n4:svazekPeriodika
91
n4:tvurceVysledku
Forrester, J. V. Kuchynka, Pavel Sosnová-Netuková, Magdaléna
n4:wos
000244425900029
s:issn
0007-1161
s:numberOfPages
7
n7:organizacniJednotka
11120