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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F07%3A00000464%21RIV08-MSM-11120___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
A 3D culture system was used to investigate the behaviour of mesothelial cells present in the wall of human processus vaginalis peritonei. Small tissue fragments placed on collagen sponges were cultured for 7, 14 and 21 days in medium supplemented with 10% FBS, and analysed for the expression and distribution of cytokeratins (CKAEI-AE3, CK19), p63, Ki-67, vimentin, CD34, and HBNM-1. Before culture, flat mesothelial cells displayed immunoreactivity for cytokeratins, vimentin and HBME-1, while p63 and CD34 were negative. Mesenchymal cells within the stroma were vimentin-positive and endothelial cells of small vessels displayed positive staining for CD34. Cytokeratins, p63 and HBME-1 were negative in all stromal cells in cultured fragments, flat mesothelial cells positive for vimentin, cytokeratins and HBME-1 proliferated, lining the fragment surface and migrating into the sponge. Capillaries showed morphological alterations; however, their immunoreactivity was comparable with the stroma prior to culture A 3D culture system was used to investigate the behaviour of mesothelial cells present in the wall of human processus vaginalis peritonei. Small tissue fragments placed on collagen sponges were cultured for 7, 14 and 21 days in medium supplemented with 10% FBS, and analysed for the expression and distribution of cytokeratins (CKAEI-AE3, CK19), p63, Ki-67, vimentin, CD34, and HBNM-1. Before culture, flat mesothelial cells displayed immunoreactivity for cytokeratins, vimentin and HBME-1, while p63 and CD34 were negative. Mesenchymal cells within the stroma were vimentin-positive and endothelial cells of small vessels displayed positive staining for CD34. Cytokeratins, p63 and HBME-1 were negative in all stromal cells in cultured fragments, flat mesothelial cells positive for vimentin, cytokeratins and HBME-1 proliferated, lining the fragment surface and migrating into the sponge. Capillaries showed morphological alterations; however, their immunoreactivity was comparable with the stroma prior to culture Autoři popisují, že expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei
dcterms:title
Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei Expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei
skos:prefLabel
Expanze HMP buněk je dlouhodobou 3D organotypickou kulturou procesus vaginalis peritonei Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei Expansion of human mesothelial progenitor cells in a long-term three-dimensional organotypic culture of Processus vaginalis peritonei
skos:notation
RIV/00216208:11120/07:00000464!RIV08-MSM-11120___
n3:strany
50-57
n3:aktivita
n11:V
n3:aktivity
V
n3:cisloPeriodika
2
n3:dodaniDat
n7:2008
n3:domaciTvurceVysledku
n6:7449186
n3:druhVysledku
n17:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
421073
n3:idVysledku
RIV/00216208:11120/07:00000464
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Processus vaginalis peritonei; mesothelial cells; CD34; cytokeratins; HBME-1
n3:klicoveSlovo
n4:mesothelial%20cells n4:CD34 n4:HBME-1 n4:Processus%20vaginalis%20peritonei n4:cytokeratins
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[CE0658CC9513]
n3:nazevZdroje
Folia Biologica
n3:obor
n9:FP
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n7:2007
n3:svazekPeriodika
53
n3:tvurceVysledku
Tanda, G. Revoltella, R. P. Rosellini, A. Mandys, Václav Michelini, M.
s:issn
0015-5500
s:numberOfPages
8
n15:organizacniJednotka
11120