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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F01%3A00003804%21RIV12-MSM-11120___
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio
dcterms:title
Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles
skos:prefLabel
Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles
skos:notation
RIV/00216208:11120/01:00003804!RIV12-MSM-11120___
n5:aktivita
n13:Z n13:P
n5:aktivity
P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(MSM 111100003)
n5:cisloPeriodika
2
n5:dodaniDat
n16:2012
n5:domaciTvurceVysledku
n12:2683350 n12:9917357 n12:4916956
n5:druhVysledku
n15:J
n5:duvernostUdaju
n8:S
n5:entitaPredkladatele
n18:predkladatel
n5:idSjednocenehoVysledku
692605
n5:idVysledku
RIV/00216208:11120/01:00003804
n5:jazykVysledku
n17:eng
n5:klicovaSlova
Pre-Messenger-Rna; Polymerase-Ii Transcription; Ribonucleoprotein-Particles; Splicing Factors; Living Cells; Nascent Transcripts; Functional U1; Sc-35 Domains; Sr Proteins; Localization
n5:klicoveSlovo
n6:Sc-35%20Domains n6:Living%20Cells n6:Nascent%20Transcripts n6:Splicing%20Factors n6:Sr%20Proteins n6:Pre-Messenger-Rna n6:Localization n6:Ribonucleoprotein-Particles n6:Polymerase-Ii%20Transcription n6:Functional%20U1
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[1DB13147DA05]
n5:nazevZdroje
Molecular Biology of the Cell
n5:obor
n14:EB
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
5
n5:projekt
n10:GA304%2F00%2F1481 n10:GA302%2F99%2F0587 n10:VS96129
n5:rokUplatneniVysledku
n16:2001
n5:svazekPeriodika
12
n5:tvurceVysledku
Melčák, Ivo Raška, Ivan Kopský, Vojtěch
n5:wos
000170348000012
n5:zamer
n19:MSM%20111100003
s:issn
1059-1524
s:numberOfPages
14
n11:organizacniJednotka
11120