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Statements

Subject Item
n2:RIV%2F00216208%3A11120%2F00%3A00003755%21RIV12-MSM-11120___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1078/S0171-9335(04)70023-0
dcterms:description
The available data concerning the subnucleolar localisation of the individual steps of precursor-ribosomal RNA (pre-rRNA) processing are ambiguous. According to in situ hybridisation studies, the late steps of pre-rRNA processing have been located into the granular component of the nucleolus, but factors engaged in these events were found being enriched in the dense fibrillar component. In this study, by utilisation of permeabilised human cells, we demonstrate that the newly synthesised, bromouridine-labelled pre-rRNAs reside at, or near, the sites of transcription. We provide evidence that processing of pre-rRNA occurs in permeabilised mammalian cells and that the incorporated bromouridine residues do not interfere with pre-rRNA maturation. Our results suggest that the maturation process of ribosomal RNA in permeabilised cells takes place at, or nearby, the site of transcription and that the processing complex is assembled during or early after the rRNA transcription. The available data concerning the subnucleolar localisation of the individual steps of precursor-ribosomal RNA (pre-rRNA) processing are ambiguous. According to in situ hybridisation studies, the late steps of pre-rRNA processing have been located into the granular component of the nucleolus, but factors engaged in these events were found being enriched in the dense fibrillar component. In this study, by utilisation of permeabilised human cells, we demonstrate that the newly synthesised, bromouridine-labelled pre-rRNAs reside at, or near, the sites of transcription. We provide evidence that processing of pre-rRNA occurs in permeabilised mammalian cells and that the incorporated bromouridine residues do not interfere with pre-rRNA maturation. Our results suggest that the maturation process of ribosomal RNA in permeabilised cells takes place at, or nearby, the site of transcription and that the processing complex is assembled during or early after the rRNA transcription.
dcterms:title
Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription
skos:prefLabel
Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription
skos:notation
RIV/00216208:11120/00:00003755!RIV12-MSM-11120___
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(AV0Z5039906)
n3:cisloPeriodika
3
n3:dodaniDat
n7:2012
n3:domaciTvurceVysledku
n16:3556654 n16:2683350
n3:druhVysledku
n9:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
722966
n3:idVysledku
RIV/00216208:11120/00:00003755
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Ribosomal RNA; processing; transcription; nucleolus; bromouridine
n3:klicoveSlovo
n10:processing n10:nucleolus n10:transcription n10:bromouridine n10:Ribosomal%20RNA
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[1CCC20F327E7]
n3:nazevZdroje
European Journal of Cell Biology
n3:obor
n11:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n12:VS96129 n12:GA302%2F99%2F0587 n12:GA304%2F00%2F1481
n3:rokUplatneniVysledku
n7:2000
n3:svazekPeriodika
79
n3:tvurceVysledku
Kiss, Tamás Staněk, David Raška, Ivan
n3:wos
000086411200006
n3:zamer
n18:AV0Z5039906
s:issn
0171-9335
s:numberOfPages
6
n21:doi
10.1078/S0171-9335(04)70023-0
n14:organizacniJednotka
11120