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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F14%3A10286366%21RIV15-MSM-11110___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://www.formatex.info/microscopy6/book/259-266.pdf
dcterms:description
The spatial organization of plasma membrane proteins such as the insulin receptor (IR) and the insulin-like growth factor receptor (IGF1R) reflect their functional relationships. In particular, the clustering or aggregation of plasma membrane proteins involved in cellular signaling is an important mechanism of cellular regulation and has long been a topic of research. Efforts to elucidate the functional relationships and the dynamic state of organization of plasma membrane molecules have historically relied on classical biochemical assays and on measurements of intermolecular interactions using methods such as fluorescence resonance energy transfer (FRET). However, using recently developed superresolution imaging methods such as single molecule localization microscopy (SMLM), we can now directly image the organization of receptors in the plasma membrane with a resolution of ~20 nm. With this data we can estimate parameters such as receptor number and density. We can also evaluate phenomena such as receptor clustering directly and efficiently. Here we present our preliminary data, imaging methods, and evaluation approaches for visualization of IR in the plasma membrane of rat basophilic leukemia (RBL)-2H3 cells. The spatial organization of plasma membrane proteins such as the insulin receptor (IR) and the insulin-like growth factor receptor (IGF1R) reflect their functional relationships. In particular, the clustering or aggregation of plasma membrane proteins involved in cellular signaling is an important mechanism of cellular regulation and has long been a topic of research. Efforts to elucidate the functional relationships and the dynamic state of organization of plasma membrane molecules have historically relied on classical biochemical assays and on measurements of intermolecular interactions using methods such as fluorescence resonance energy transfer (FRET). However, using recently developed superresolution imaging methods such as single molecule localization microscopy (SMLM), we can now directly image the organization of receptors in the plasma membrane with a resolution of ~20 nm. With this data we can estimate parameters such as receptor number and density. We can also evaluate phenomena such as receptor clustering directly and efficiently. Here we present our preliminary data, imaging methods, and evaluation approaches for visualization of IR in the plasma membrane of rat basophilic leukemia (RBL)-2H3 cells.
dcterms:title
Imaging of insulin receptors in the plasma membrane of cells using superresolution single molecule localization microscopy Imaging of insulin receptors in the plasma membrane of cells using superresolution single molecule localization microscopy
skos:prefLabel
Imaging of insulin receptors in the plasma membrane of cells using superresolution single molecule localization microscopy Imaging of insulin receptors in the plasma membrane of cells using superresolution single molecule localization microscopy
skos:notation
RIV/00216208:11110/14:10286366!RIV15-MSM-11110___
n3:aktivita
n18:I n18:P
n3:aktivity
I, P(ED1.1.00/02.0109), P(GBP302/12/G157), P(GP14-15272P), P(GPP205/12/P392)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n9:5606276 n9:8242321 n9:2095270 n9:8101396 n9:6675581
n3:druhVysledku
n6:C
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
20493
n3:idVysledku
RIV/00216208:11110/14:10286366
n3:jazykVysledku
n7:eng
n3:klicovaSlova
STORM; PALM; single molecule; super-resolution microscopy; receptor clustering; cancer; diabetes; insulin
n3:klicoveSlovo
n4:cancer n4:super-resolution%20microscopy n4:STORM n4:receptor%20clustering n4:insulin n4:single%20molecule n4:diabetes n4:PALM
n3:kontrolniKodProRIV
[2877C8061225]
n3:mistoVydani
Badajoz
n3:nazevEdiceCisloSvazku
Microscopy Book Series #6, 1
n3:nazevZdroje
Microscopy: advances in scientific research and education
n3:obor
n5:EA
n3:pocetDomacichTvurcuVysledku
5
n3:pocetStranKnihy
588
n3:pocetTvurcuVysledku
8
n3:projekt
n14:GBP302%2F12%2FG157 n14:ED1.1.00%2F02.0109 n14:GPP205%2F12%2FP392 n14:GP14-15272P
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Winter, Peter W. Křížek, Pavel Hagen, Guy Michael Ovesný, Martin Roess, Deborah A. Borkovec, Josef Švindrych, Zdeněk Barisas, George B.
s:numberOfPages
8
n21:hasPublisher
Formatex Research Center
n11:isbn
978-84-942134-3-4
n16:organizacniJednotka
11110