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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F14%3A10285970%21RIV15-MSM-11110___
rdf:type
skos:Concept n16:Vysledek
rdfs:seeAlso
http://fb.cuni.cz/file/5718/FB2014A0010.pdf
dcterms:description
The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The maturation state (presence of CD83) of cord blood monocyte-derived dendritic cells (moDCs) 01 52 children of healthy mothers and 58 children of allergic mothers was estimated by flow cytometry. The capacity of moDCs to express genes for subunits of IL-12 family cytokines was monitored using real-time PCR and protein secretion in cell culture supernatants by ELISA. The percentage of CD83(+) moDCs was significantly higher in the allergic group after LPS stimulation (43.11 +/- 4.41) in comparison to the healthy group (24.85 +/- 3.37). Significantly higher gene expression of subunits of IL-12 family members was observed in moDCs of children of allergic mothers, in comparison with children of healthy mothers. The differences were evident mainly after LPS stimulation of moDCs (healthy group: p19: 3.05 +/- 1.24; p28: 14.8 +/- 6.8;p35: 1.8 +/- 0.6; p40: 8.0 +/- 3.5; EBI3: 3.0 +/- 1.2; allergic group: p19: 6.1 +/- 2.7; p28: 61.4 +/- 22.2; p35: 14.9 +/- 6.5; p40: 36.4 +/- 18.8; EBI3: 11.3 +/- 3.2), with the exception of p28, whose expression was significantly higher in the allergic group even without stimulation (healthy group: 0.28 +/- 0.12, allergic group: 0.87 +/- 0.62). No significant difference between the healthy and allergic groups was found at the protein level. The observation of both increased presence of cell surface activation marker on moDCs and higher IL-12 family gene expression in LPS-stimulated moDCs of children of allergic mothers indicates a higher reactivity of these cells. The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The maturation state (presence of CD83) of cord blood monocyte-derived dendritic cells (moDCs) 01 52 children of healthy mothers and 58 children of allergic mothers was estimated by flow cytometry. The capacity of moDCs to express genes for subunits of IL-12 family cytokines was monitored using real-time PCR and protein secretion in cell culture supernatants by ELISA. The percentage of CD83(+) moDCs was significantly higher in the allergic group after LPS stimulation (43.11 +/- 4.41) in comparison to the healthy group (24.85 +/- 3.37). Significantly higher gene expression of subunits of IL-12 family members was observed in moDCs of children of allergic mothers, in comparison with children of healthy mothers. The differences were evident mainly after LPS stimulation of moDCs (healthy group: p19: 3.05 +/- 1.24; p28: 14.8 +/- 6.8;p35: 1.8 +/- 0.6; p40: 8.0 +/- 3.5; EBI3: 3.0 +/- 1.2; allergic group: p19: 6.1 +/- 2.7; p28: 61.4 +/- 22.2; p35: 14.9 +/- 6.5; p40: 36.4 +/- 18.8; EBI3: 11.3 +/- 3.2), with the exception of p28, whose expression was significantly higher in the allergic group even without stimulation (healthy group: 0.28 +/- 0.12, allergic group: 0.87 +/- 0.62). No significant difference between the healthy and allergic groups was found at the protein level. The observation of both increased presence of cell surface activation marker on moDCs and higher IL-12 family gene expression in LPS-stimulated moDCs of children of allergic mothers indicates a higher reactivity of these cells.
dcterms:title
Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers
skos:prefLabel
Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers
skos:notation
RIV/00216208:11110/14:10285970!RIV15-MSM-11110___
n4:aktivita
n7:P n7:I
n4:aktivity
I, P(GD310/08/H077)
n4:cisloPeriodika
2
n4:dodaniDat
n12:2015
n4:domaciTvurceVysledku
n11:5123372 n11:4263170 n11:9878084
n4:druhVysledku
n10:J
n4:duvernostUdaju
n13:S
n4:entitaPredkladatele
n8:predkladatel
n4:idSjednocenehoVysledku
18009
n4:idVysledku
RIV/00216208:11110/14:10285970
n4:jazykVysledku
n19:eng
n4:klicovaSlova
dendritic cells; cord blood; allergy; gene expression; IL-12 family; CD83
n4:klicoveSlovo
n5:IL-12%20family n5:allergy n5:CD83 n5:cord%20blood n5:gene%20expression n5:dendritic%20cells
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[158EF4EF2AF3]
n4:nazevZdroje
Folia Biologica
n4:obor
n18:EC
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n14:GD310%2F08%2FH077
n4:rokUplatneniVysledku
n12:2014
n4:svazekPeriodika
60
n4:tvurceVysledku
Prokešová, Ludmila Hrdý, Jiří Kocourková, Ivana Novotná, Olga
n4:wos
000334986300004
s:issn
0015-5500
s:numberOfPages
9
n15:organizacniJednotka
11110