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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F14%3A10285448%21RIV15-MSM-11110___
rdf:type
skos:Concept n19:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1007/s00726-014-1700-8
dcterms:description
The oxidation of cysteine in mammalian cells occurs by two routes: a highly regulated direct oxidation pathway in which the first step is catalyzed by cysteine dioxygenase (CDO) and by desulfhydration-oxidation pathways in which the sulfur is released in a reduced oxidation state. To assess the effect of a lack of CDO on production of hydrogen sulfide (H2S) and thiosulfate (an intermediate in the oxidation of H2S to sulfate) and to explore the roles of both cystathionine gamma-lyase (CTH) and cystathionine beta-synthase (CBS) in cysteine desulfhydration by liver, we investigated the metabolism of cysteine in hepatocytes isolated from Cdo1-null and wild-type mice. Hepatocytes from Cdo1-null mice produced more H2S and thiosulfate than did hepatocytes from wild-type mice. The greater flux of cysteine through the cysteine desulfhydration reactions catalyzed by CTH and CBS in hepatocytes from Cdo1-null mice appeared to be the consequence of their higher cysteine levels, which were due to the lack of CDO and hence lack of catabolism of cysteine by the cysteinesulfinate-dependent pathways. Both CBS and CTH appeared to contribute substantially to cysteine desulfhydration, with estimates of 56 % by CBS and 44 % by CTH in hepatocytes from wild-type mice, and 63 % by CBS and 37 % by CTH in hepatocytes from Cdo1-null mice. The oxidation of cysteine in mammalian cells occurs by two routes: a highly regulated direct oxidation pathway in which the first step is catalyzed by cysteine dioxygenase (CDO) and by desulfhydration-oxidation pathways in which the sulfur is released in a reduced oxidation state. To assess the effect of a lack of CDO on production of hydrogen sulfide (H2S) and thiosulfate (an intermediate in the oxidation of H2S to sulfate) and to explore the roles of both cystathionine gamma-lyase (CTH) and cystathionine beta-synthase (CBS) in cysteine desulfhydration by liver, we investigated the metabolism of cysteine in hepatocytes isolated from Cdo1-null and wild-type mice. Hepatocytes from Cdo1-null mice produced more H2S and thiosulfate than did hepatocytes from wild-type mice. The greater flux of cysteine through the cysteine desulfhydration reactions catalyzed by CTH and CBS in hepatocytes from Cdo1-null mice appeared to be the consequence of their higher cysteine levels, which were due to the lack of CDO and hence lack of catabolism of cysteine by the cysteinesulfinate-dependent pathways. Both CBS and CTH appeared to contribute substantially to cysteine desulfhydration, with estimates of 56 % by CBS and 44 % by CTH in hepatocytes from wild-type mice, and 63 % by CBS and 37 % by CTH in hepatocytes from Cdo1-null mice.
dcterms:title
Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate
skos:prefLabel
Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate
skos:notation
RIV/00216208:11110/14:10285448!RIV15-MSM-11110___
n3:aktivita
n10:I
n3:aktivity
I
n3:cisloPeriodika
5
n3:dodaniDat
n7:2015
n3:domaciTvurceVysledku
n5:9185283
n3:druhVysledku
n13:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
39344
n3:idVysledku
RIV/00216208:11110/14:10285448
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Hepatocytes; Mice; Thiosulfate; Hydrogen sulfide; Cystathionine beta-synthase; Cystathionine gamma-lyase; Cysteine dioxygenase; Cysteine
n3:klicoveSlovo
n11:Cystathionine%20beta-synthase n11:Cystathionine%20gamma-lyase n11:Cysteine n11:Hepatocytes n11:Cysteine%20dioxygenase n11:Hydrogen%20sulfide n11:Mice n11:Thiosulfate
n3:kodStatuVydavatele
AT - Rakouská republika
n3:kontrolniKodProRIV
[EED08D43BFE8]
n3:nazevZdroje
Amino Acids
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n7:2014
n3:svazekPeriodika
46
n3:tvurceVysledku
Roman, Heather B. Hanaoka, Kenjiro Sasakura, Kiyoshi Hirschberger, Lawrence L. Nagano, Tetsuo Jurkowska, Halina Stipanuk, Martha H. Krijt, Jakub
n3:wos
000334516700022
s:issn
0939-4451
s:numberOfPages
13
n12:doi
10.1007/s00726-014-1700-8
n6:organizacniJednotka
11110