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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F14%3A10284132%21RIV15-MSM-11110___
rdf:type
skos:Concept n19:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1016/j.cca.2014.07.028
dcterms:description
Background: Disorders of homocysteine and B-vitamin metabolism represent a significant problem in clinical practice. Establishing the diagnosis requires specialized tests with demanding preanalytical requirements. To advance the detection of patients with these disorders, we developed a method for the simultaneous determination of cystathionine (Cysta), methionine (Met) and total homocysteine (tHcy) in dried blood spots (DBSs). Methods: A punch from a DBS sample was mixed with a solution of isotopically labeled internal standards, and analytes were extracted using methanol/0.1% formic acid/0.5 mol/L dithiothreitol. The extract was injected into an LC-MS/MS system operating in MRM mode. Results: The analytical performance of the method employing DBS is adequate for its purpose and the type of sample. Compared with Cysta, tHcy and Met plasma levels, our method exhibited a negative bias between -3.8% and -42.2% due to the lower concentrations of these analytes in erythrocytes. The tHcy level and the Met/Cysta ratio in DBS enabled the clear detection of 12 patients with disorders of transsulfuration and with genetic and nutritional remethylation defects. Conclusions: The ease of collecting and transporting DBS samples may advance diagnostic procedures in patients with neuropsychiatric disorders and thromboembolism. Consequently, this approach may facilitate detection and simplify the monitoring of patients with homocystinuria. Background: Disorders of homocysteine and B-vitamin metabolism represent a significant problem in clinical practice. Establishing the diagnosis requires specialized tests with demanding preanalytical requirements. To advance the detection of patients with these disorders, we developed a method for the simultaneous determination of cystathionine (Cysta), methionine (Met) and total homocysteine (tHcy) in dried blood spots (DBSs). Methods: A punch from a DBS sample was mixed with a solution of isotopically labeled internal standards, and analytes were extracted using methanol/0.1% formic acid/0.5 mol/L dithiothreitol. The extract was injected into an LC-MS/MS system operating in MRM mode. Results: The analytical performance of the method employing DBS is adequate for its purpose and the type of sample. Compared with Cysta, tHcy and Met plasma levels, our method exhibited a negative bias between -3.8% and -42.2% due to the lower concentrations of these analytes in erythrocytes. The tHcy level and the Met/Cysta ratio in DBS enabled the clear detection of 12 patients with disorders of transsulfuration and with genetic and nutritional remethylation defects. Conclusions: The ease of collecting and transporting DBS samples may advance diagnostic procedures in patients with neuropsychiatric disorders and thromboembolism. Consequently, this approach may facilitate detection and simplify the monitoring of patients with homocystinuria.
dcterms:title
Simultaneous determination of cystathionine, total homocysteine, and methionine in dried blood spots by liquid chromatography/tandem mass spectrometry and its utility for the management of patients with homocystinuria Simultaneous determination of cystathionine, total homocysteine, and methionine in dried blood spots by liquid chromatography/tandem mass spectrometry and its utility for the management of patients with homocystinuria
skos:prefLabel
Simultaneous determination of cystathionine, total homocysteine, and methionine in dried blood spots by liquid chromatography/tandem mass spectrometry and its utility for the management of patients with homocystinuria Simultaneous determination of cystathionine, total homocysteine, and methionine in dried blood spots by liquid chromatography/tandem mass spectrometry and its utility for the management of patients with homocystinuria
skos:notation
RIV/00216208:11110/14:10284132!RIV15-MSM-11110___
n4:aktivita
n16:I
n4:aktivity
I
n4:cisloPeriodika
November
n4:dodaniDat
n8:2015
n4:domaciTvurceVysledku
n12:3469964 n12:9185283 n12:3950131
n4:druhVysledku
n13:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
45045
n4:idVysledku
RIV/00216208:11110/14:10284132
n4:jazykVysledku
n18:eng
n4:klicovaSlova
Therapy monitoring; LC-MS/MS; Dried blood spots; Methionine; Homocysteine; Cystathionine
n4:klicoveSlovo
n5:Therapy%20monitoring n5:Homocysteine n5:Dried%20blood%20spots n5:LC-MS%2FMS n5:Cystathionine n5:Methionine
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[C16D47798A62]
n4:nazevZdroje
Clinica Chimica Acta
n4:obor
n17:FB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n8:2014
n4:svazekPeriodika
437
n4:tvurceVysledku
Hodík, Jakub Bártl, Josef Krijt, Jakub Kožich, Viktor Pešková, Karolína Chrastina, Petr
n4:wos
000342246900036
s:issn
0009-8981
s:numberOfPages
7
n7:doi
10.1016/j.cca.2014.07.028
n15:organizacniJednotka
11110