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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F13%3A10194761%21RIV14-GA0-11110___
rdf:type
skos:Concept n9:Vysledek
rdfs:seeAlso
http://link.springer.com/protocol/10.1007%2F978-1-62703-526-2_11#
dcterms:description
During development, the genome undergoes drastic reorganization within the nuclear space. To determine tridimensional genome folding, genome-wide techniques (damID/Hi-C) can be applied using cell populations, but these have to be calibrated using microscopy and single-cell analysis of gene positioning. Moreover, the dynamic behavior of chromatin has to be assessed on living samples. Combining fast stereotypic development with easy genetics and microscopy, the nematode C. elegans has become a model of choice in recent years to study changes in nuclear organization during cell fate acquisition. Here we present two complementary techniques to evaluate nuclear positioning of genes either by fluorescence in situ hybridization in fixed samples or in living worm embryos using the GFP-lacI/lacO chromatin-tagging system. During development, the genome undergoes drastic reorganization within the nuclear space. To determine tridimensional genome folding, genome-wide techniques (damID/Hi-C) can be applied using cell populations, but these have to be calibrated using microscopy and single-cell analysis of gene positioning. Moreover, the dynamic behavior of chromatin has to be assessed on living samples. Combining fast stereotypic development with easy genetics and microscopy, the nematode C. elegans has become a model of choice in recent years to study changes in nuclear organization during cell fate acquisition. Here we present two complementary techniques to evaluate nuclear positioning of genes either by fluorescence in situ hybridization in fixed samples or in living worm embryos using the GFP-lacI/lacO chromatin-tagging system.
dcterms:title
Microscopic analysis of chromatin localization and dynamics in C. elegans Microscopic analysis of chromatin localization and dynamics in C. elegans
skos:prefLabel
Microscopic analysis of chromatin localization and dynamics in C. elegans Microscopic analysis of chromatin localization and dynamics in C. elegans
skos:notation
RIV/00216208:11110/13:10194761!RIV14-GA0-11110___
n9:predkladatel
n11:orjk%3A11110
n4:aktivita
n6:I n6:P
n4:aktivity
I, P(GAP302/11/1262), P(GBP302/12/G157)
n4:dodaniDat
n18:2014
n4:domaciTvurceVysledku
Lanctôt, Christian
n4:druhVysledku
n21:C
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
88211
n4:idVysledku
RIV/00216208:11110/13:10194761
n4:jazykVysledku
n5:eng
n4:klicovaSlova
Microscopy; GFP-lacI/lacO; Chromatin tagging; Fluorescence in situ hybridization; C. elegans; Nuclear organization
n4:klicoveSlovo
n7:GFP-lacI%2FlacO n7:Chromatin%20tagging n7:Microscopy n7:Fluorescence%20in%20situ%20hybridization n7:C.%20elegans n7:Nuclear%20organization
n4:kontrolniKodProRIV
[83FA3185FBC7]
n4:mistoVydani
New York, NY, USA
n4:nazevEdiceCisloSvazku
1042
n4:nazevZdroje
Imaging Gene Expression
n4:obor
n20:EB
n4:pocetDomacichTvurcuVysledku
1
n4:pocetStranKnihy
368
n4:pocetTvurcuVysledku
2
n4:projekt
n12:GBP302%2F12%2FG157 n12:GAP302%2F11%2F1262
n4:rokUplatneniVysledku
n18:2013
n4:tvurceVysledku
Meister, Peter Lanctôt, Christian
s:numberOfPages
20
n14:doi
10.1007/978-1-62703-526-2_11
n13:hasPublisher
Humana Press
n19:isbn
978-1-62703-525-5
n22:organizacniJednotka
11110