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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F13%3A10190092%21RIV14-GA0-11110___
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1021/pr300980v
dcterms:description
An example of bacterium, which undergoes a complex development, is the genus of Streptomyces whose importance lies in their wide capacity to produce secondary metabolites, including antibiotics. In this work, a proteomic approach was applied to the systems study of germination as a transition from dormancy to the metabolically active stage. The protein expression levels were examined throughout the germination time course, the kinetics of the accumulated and newly synthesized proteins were clustered, and proteins detected in each group were identified. Altogether, 104 2DE gel images at 13 time points, from dormant state until 5.5 h of growth, were analyzed. The mass spectrometry identified proteins were separated into functional groups and their potential roles during germination were further assessed. The showed that the full competence of spores to effectively undergo active metabolism is derived from the sporulation step, which facilitates the rapid initiation of global protein expression during the first 10 min of cultivation. Within the first hour, the majority of proteins were synthesized. From this stage, the full capability of regulatory mechanisms to respond to environmental cues is presumed. The obtained results might also provide a data source for further investigations of the process of germination. An example of bacterium, which undergoes a complex development, is the genus of Streptomyces whose importance lies in their wide capacity to produce secondary metabolites, including antibiotics. In this work, a proteomic approach was applied to the systems study of germination as a transition from dormancy to the metabolically active stage. The protein expression levels were examined throughout the germination time course, the kinetics of the accumulated and newly synthesized proteins were clustered, and proteins detected in each group were identified. Altogether, 104 2DE gel images at 13 time points, from dormant state until 5.5 h of growth, were analyzed. The mass spectrometry identified proteins were separated into functional groups and their potential roles during germination were further assessed. The showed that the full competence of spores to effectively undergo active metabolism is derived from the sporulation step, which facilitates the rapid initiation of global protein expression during the first 10 min of cultivation. Within the first hour, the majority of proteins were synthesized. From this stage, the full capability of regulatory mechanisms to respond to environmental cues is presumed. The obtained results might also provide a data source for further investigations of the process of germination.
dcterms:title
Systems Insight into the Spore Germination of Streptomyces coelicolor Systems Insight into the Spore Germination of Streptomyces coelicolor
skos:prefLabel
Systems Insight into the Spore Germination of Streptomyces coelicolor Systems Insight into the Spore Germination of Streptomyces coelicolor
skos:notation
RIV/00216208:11110/13:10190092!RIV14-GA0-11110___
n13:predkladatel
n20:orjk%3A11110
n3:aktivita
n10:S n10:P n10:I
n3:aktivity
I, P(GA310/07/1009), P(GAP302/10/0468), P(GAP302/11/0229), S
n3:cisloPeriodika
1
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
n14:2280639
n3:druhVysledku
n15:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
109713
n3:idVysledku
RIV/00216208:11110/13:10190092
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Streptomyces; protein expression; differentiation; germination
n3:klicoveSlovo
n4:germination n4:Streptomyces n4:protein%20expression n4:differentiation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[39B36ED2E229]
n3:nazevZdroje
Journal of Proteome Research
n3:obor
n8:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:projekt
n5:GAP302%2F10%2F0468 n5:GA310%2F07%2F1009 n5:GAP302%2F11%2F0229
n3:rokUplatneniVysledku
n16:2013
n3:svazekPeriodika
12
n3:tvurceVysledku
Rehulka, Pavel Rehulkova, Helena Benada, Oldřich Kofronova, Olga Vohradsky, Jiří Strakova, Eva Bobek, Jan Zikova, Alice
n3:wos
000313156300052
s:issn
1535-3893
s:numberOfPages
12
n21:doi
10.1021/pr300980v
n18:organizacniJednotka
11110