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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F11%3A10546%21RIV12-MSM-11110___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.jplph.2010.10.014
dcterms:description
After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. The architecture of the well-organized nuclear functional domains changes in response to DNA replication, RNA transcription, processing and transport dynamics. A number of subnuclear structures present in the interchromatin region (IR, the nuclear domain between chromosome territories) have been shown as involved, either directly or indirectly, in transcriptional regulation. These structures include the interchromatin granule clusters (IGCs), perichromatin fibrils (PFs), Cajal bodies (CBs) and perichromatin granules (PGs). In this work, we present a cytochemical, immunocytochemical, quantitative and morphometric analysis at the light, confocal and electron microscopy levels to characterize the changes in the functional architecture of the nuclear interchromatin domain during two developmental programs followed by the microspore: differentiation to mature pollen grains (transcriptionally inactive), and microspore embryogenesis involving proliferation in the first stages (highly engaged in transcription). Our results revealed characteristic changes in size, shape and distribution of the different interchromatin structures as a consequence of the reprogramming of the microspore, allowing us to relate the remodeling of the interchromatin domain to the variations in transcriptional activities during proliferation and differentiation events, and suggesting that RNA-associated structures could be a regulatory mechanism in the process. In addition, we document the presence of two structurally different types of CBs, and of IGC and CB-associated regions, similar to those present in animal cells, and not yet described in plants. After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. The architecture of the well-organized nuclear functional domains changes in response to DNA replication, RNA transcription, processing and transport dynamics. A number of subnuclear structures present in the interchromatin region (IR, the nuclear domain between chromosome territories) have been shown as involved, either directly or indirectly, in transcriptional regulation. These structures include the interchromatin granule clusters (IGCs), perichromatin fibrils (PFs), Cajal bodies (CBs) and perichromatin granules (PGs). In this work, we present a cytochemical, immunocytochemical, quantitative and morphometric analysis at the light, confocal and electron microscopy levels to characterize the changes in the functional architecture of the nuclear interchromatin domain during two developmental programs followed by the microspore: differentiation to mature pollen grains (transcriptionally inactive), and microspore embryogenesis involving proliferation in the first stages (highly engaged in transcription). Our results revealed characteristic changes in size, shape and distribution of the different interchromatin structures as a consequence of the reprogramming of the microspore, allowing us to relate the remodeling of the interchromatin domain to the variations in transcriptional activities during proliferation and differentiation events, and suggesting that RNA-associated structures could be a regulatory mechanism in the process. In addition, we document the presence of two structurally different types of CBs, and of IGC and CB-associated regions, similar to those present in animal cells, and not yet described in plants.
dcterms:title
A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L. A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L.
skos:prefLabel
A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L. A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L.
skos:notation
RIV/00216208:11110/11:10546!RIV12-MSM-11110___
n5:predkladatel
n17:orjk%3A11110
n3:aktivita
n15:Z n15:P
n3:aktivity
P(LC535), Z(MSM0021620806)
n3:cisloPeriodika
8
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n19:2683350
n3:druhVysledku
n21:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
183655
n3:idVysledku
RIV/00216208:11110/11:10546
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Cajal bodies; Differentiation; Maturation; Microspore embryogenesis; Nucleus; Pollen; Proliferation; Rapeseed
n3:klicoveSlovo
n4:Proliferation n4:Rapeseed n4:Differentiation n4:Nucleus n4:Microspore%20embryogenesis n4:Cajal%20bodies n4:Maturation n4:Pollen
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[1288A1B2789E]
n3:nazevZdroje
Journal of Plant Physiology
n3:obor
n14:EA
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n18:LC535
n3:rokUplatneniVysledku
n9:2011
n3:svazekPeriodika
168
n3:tvurceVysledku
Seguí-Simarro, J. M. Corredor, E. Testillano, P. S. Raška, Ivan Risueno, M. C. Corral-Martinez, P.
n3:wos
000290884800002
n3:zamer
n8:MSM0021620806
s:issn
0176-1617
s:numberOfPages
12
n16:organizacniJednotka
11110