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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F09%3A4920%21RIV10-GA0-11110___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Lx mutation in SHR.Lx rat manifests as hindlimb preaxial polydactyly. Lx was previously mapped to a chromosome 8 segment containing the Plzf gene. However, the Plzf coding sequence is intact in mutants. Using linkage mapping in F2 hybrids, we downsized the segment containing Lx to 155 kb and sequenced conserved noncoding elements (CNEs) inside. A 2,964-bp deletion in Plzf intron 2, never detected in control animals, is the only candidate for Lx. The deletion removes the most deeply conserved CNE in the 155-kb segment, suggesting a regulatory influence on Plzf expression. Correspondingly, using in situ hybridization and quantitative real-time polymerase chain reaction, we found a decrease of Plzf expression in Lx/Lx limb buds with concomitant anterior expansion of expression domains of its targets, Hoxd10-13 genes, in the absence of ectopic Sonic hedgehog expression. Upstream regulation of Plzf in limb buds is currently unknown. We present here the first candidate Plzf cis-regulatory sequence. Lx mutation in SHR.Lx rat manifests as hindlimb preaxial polydactyly. Lx was previously mapped to a chromosome 8 segment containing the Plzf gene. However, the Plzf coding sequence is intact in mutants. Using linkage mapping in F2 hybrids, we downsized the segment containing Lx to 155 kb and sequenced conserved noncoding elements (CNEs) inside. A 2,964-bp deletion in Plzf intron 2, never detected in control animals, is the only candidate for Lx. The deletion removes the most deeply conserved CNE in the 155-kb segment, suggesting a regulatory influence on Plzf expression. Correspondingly, using in situ hybridization and quantitative real-time polymerase chain reaction, we found a decrease of Plzf expression in Lx/Lx limb buds with concomitant anterior expansion of expression domains of its targets, Hoxd10-13 genes, in the absence of ectopic Sonic hedgehog expression. Upstream regulation of Plzf in limb buds is currently unknown. We present here the first candidate Plzf cis-regulatory sequence.
dcterms:title
Deletion of a Conserved Noncoding Sequence in Plzf Intron Leads to Plzf Down-regulation in Limb Bud and Polydactyly in the Rat Deletion of a Conserved Noncoding Sequence in Plzf Intron Leads to Plzf Down-regulation in Limb Bud and Polydactyly in the Rat
skos:prefLabel
Deletion of a Conserved Noncoding Sequence in Plzf Intron Leads to Plzf Down-regulation in Limb Bud and Polydactyly in the Rat Deletion of a Conserved Noncoding Sequence in Plzf Intron Leads to Plzf Down-regulation in Limb Bud and Polydactyly in the Rat
skos:notation
RIV/00216208:11110/09:4920!RIV10-GA0-11110___
n3:aktivita
n5:P n5:Z
n3:aktivity
P(GA304/06/0116), P(GP301/07/P178), Z(AV0Z50110509), Z(AV0Z50450515), Z(MSM0021620806)
n3:cisloPeriodika
3
n3:dodaniDat
n16:2010
n3:domaciTvurceVysledku
n9:7556306 n9:2062402 n9:6503586 n9:1153935 n9:4490010 n9:5754623 n9:9173293 n9:1565494 n9:8558043 n9:8026610
n3:druhVysledku
n7:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
309359
n3:idVysledku
RIV/00216208:11110/09:4920
n3:jazykVysledku
n18:eng
n3:klicovaSlova
promyelocytic leukemia zinc finger (Plzf); HoxD genes; conserved noncoding element; polydactyly; zinc-finger protein; acute promyelocytic leukemia; interaction motif; congenic strains; genetic-analysis; dna-binding; poz-domain; expression; receptor; alpha
n3:klicoveSlovo
n4:expression n4:receptor n4:HoxD%20genes n4:conserved%20noncoding%20element n4:dna-binding n4:promyelocytic%20leukemia%20zinc%20finger%20%28Plzf%29 n4:congenic%20strains n4:alpha n4:poz-domain n4:zinc-finger%20protein n4:polydactyly n4:interaction%20motif n4:genetic-analysis n4:acute%20promyelocytic%20leukemia
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[51F5427E2296]
n3:nazevZdroje
Developmental Dynamics
n3:obor
n10:EB
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
11
n3:projekt
n14:GP301%2F07%2FP178 n14:GA304%2F06%2F0116
n3:rokUplatneniVysledku
n16:2009
n3:svazekPeriodika
238
n3:tvurceVysledku
Šeda, Ondřej Slámová, P. Krejčí, Eliška Šnajdr, Pavel Křen, Vladimír Sedmera, David Křenová, Drahomíra Liška, František Chylíková, Blanka Grim, Miloš Šedová, Lucie
n3:wos
000264001300016
n3:zamer
n15:MSM0021620806 n15:AV0Z50110509 n15:AV0Z50450515
s:issn
1058-8388
s:numberOfPages
12
n17:organizacniJednotka
11110