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Statements

Subject Item
n2:RIV%2F00216208%3A11110%2F09%3A4121%21RIV10-MSM-11110___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
We present a rapid and sensitive LC-MS/MS method for SAM and SAH determination in mice tissues. The method is based on chromatographic separation on a Hypercarb column filled with porous graphitic carbon stationary phase. Sufficient retention of SAM and SAH on the chromatographic packing allows simple sample preparation protocol avoiding solid phase extraction step. No significant matrix effects were observed by analysing the tissue extracts on LC-MS/MS. We present a rapid and sensitive LC-MS/MS method for SAM and SAH determination in mice tissues. The method is based on chromatographic separation on a Hypercarb column filled with porous graphitic carbon stationary phase. Sufficient retention of SAM and SAH on the chromatographic packing allows simple sample preparation protocol avoiding solid phase extraction step. No significant matrix effects were observed by analysing the tissue extracts on LC-MS/MS.
dcterms:title
Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues
skos:prefLabel
Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues
skos:notation
RIV/00216208:11110/09:4121!RIV10-MSM-11110___
n3:aktivita
n13:Z
n3:aktivity
Z(MSM0021620806)
n3:cisloPeriodika
22
n3:dodaniDat
n7:2010
n3:domaciTvurceVysledku
n9:3950131 n9:9185283
n3:druhVysledku
n8:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
309936
n3:idVysledku
RIV/00216208:11110/09:4121
n3:jazykVysledku
n18:eng
n3:klicovaSlova
S-Adenosylmethionine; S-Adenosylhomocysteine; Mass spectrometry; Liquid chromatography; Mouse tissues; cystathionine beta-synthase; performance liquid-chromatography; tandem mass-spectrometry; adenosyl-l-methionine; plasma homocysteine; cerebrospinal-fluid; methylation ratio; deficient; mouse; hydrolase
n3:klicoveSlovo
n4:S-Adenosylhomocysteine n4:Mouse%20tissues n4:performance%20liquid-chromatography n4:hydrolase n4:cerebrospinal-fluid n4:S-Adenosylmethionine n4:Liquid%20chromatography n4:tandem%20mass-spectrometry n4:plasma%20homocysteine n4:Mass%20spectrometry n4:deficient n4:cystathionine%20beta-synthase n4:mouse n4:methylation%20ratio n4:adenosyl-l-methionine
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[3AB1F02CBD98]
n3:nazevZdroje
Journal of Chromatography B
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n7:2009
n3:svazekPeriodika
877
n3:tvurceVysledku
Krijt, Jakub Duta, A. Kožich, Viktor
n3:wos
000268040400012
n3:zamer
n17:MSM0021620806
s:issn
1570-0232
s:numberOfPages
6
n16:organizacniJednotka
11110