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Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F14%3A%230000567%21RIV15-GA0-00209805
rdf:type
skos:Concept n18:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1016/j.electacta.2014.10.125
dcterms:description
His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, a-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedicine. His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, a-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedicine.
dcterms:title
Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation
skos:prefLabel
Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation
skos:notation
RIV/00209805:_____/14:#0000567!RIV15-GA0-00209805
n3:aktivita
n15:I n15:P
n3:aktivity
I, P(ED2.1.00/03.0101), P(GA13-00956S)
n3:cisloPeriodika
4 November
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n4:1330586 n4:9991646 n4:9637419 n4:6505368
n3:druhVysledku
n13:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
13332
n3:idVysledku
RIV/00209805:_____/14:#0000567
n3:jazykVysledku
n19:eng
n3:klicovaSlova
anterior gradient 2 oncoprotein; His-tagged proteins; carbon electrode; histidine; tyrosine and tryptophan oxidation
n3:klicoveSlovo
n9:carbon%20electrode n9:anterior%20gradient%202%20oncoprotein n9:histidine n9:His-tagged%20proteins n9:tyrosine%20and%20tryptophan%20oxidation
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[B3151DCC3F38]
n3:nazevZdroje
Electrochimica acta
n3:obor
n8:CG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n6:ED2.1.00%2F03.0101 n6:GA13-00956S
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
150
n3:tvurceVysledku
Ďurech, Michal Hrstka, Roman Paleček, Emil Vojtěšek, Bořivoj
n3:wos
000346185000028
s:issn
0013-4686
s:numberOfPages
5
n16:doi
10.1016/j.electacta.2014.10.125