This HTML5 document contains 49 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://linked.opendata.cz/resource/domain/vavai/projekt/
n8http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n17http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n14http://bibframe.org/vocab/
n15http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00209805%3A_____%2F14%3A%230000564%21RIV15-GA0-00209805/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n9http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n19http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n11http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F14%3A%230000564%21RIV15-GA0-00209805
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/elan.201400449/abstract
dcterms:description
Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules. Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules.
dcterms:title
Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples
skos:prefLabel
Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples Adsorptive transfer stripping for quick electrochemical determination of microRNAs in total RNA samples
skos:notation
RIV/00209805:_____/14:#0000564!RIV15-GA0-00209805
n3:aktivita
n7:I n7:P
n3:aktivity
I, P(ED2.1.00/03.0101), P(GA13-00956S), P(GP14-24931P)
n3:cisloPeriodika
October 31
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
Bartošík, Martin n8:9637419 n8:1330586 n8:9991646
n3:druhVysledku
n6:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
1713
n3:idVysledku
RIV/00209805:_____/14:#0000564
n3:jazykVysledku
n19:eng
n3:klicovaSlova
cancer; mercury electrode; microRNA; voltammetry
n3:klicoveSlovo
n9:voltammetry n9:mercury%20electrode n9:cancer n9:microRNA
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[BCA9D96A8630]
n3:nazevZdroje
Electroanalysis
n3:obor
n18:CG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n13:ED2.1.00%2F03.0101 n13:GA13-00956S n13:GP14-24931P
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
26
n3:tvurceVysledku
Vojtěšek, Bořivoj Hrstka, Roman Bartošík, Martin Paleček, Emil
n3:wos
000345978300002
s:issn
1040-0397
s:numberOfPages
5
n14:doi
10.1002/elan.201400449