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Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F13%3A%230000472%21RIV14-MZ0-00209805
rdf:type
skos:Concept n18:Vysledek
rdfs:seeAlso
http://www.tandfonline.com/doi/abs/10.1080/01635581.2013.756530#.U2EN1KJaKec
dcterms:description
Sporadic colorectal cancer (CRC) is a typical multifactorial disease. Isothiocyanates (ITC) have been recently shown to inhibit development of CRC in many experimental models. MicroRNAs (miRNAs) are short noncoding RNAs that posttranscriptionally regulate gene expression through binding to 3 untranslated regions (3UTR) of target mRNAs. MiRNAs are regulated by natural agents, ITCs included. In our study, using global expression profiling based on TaqMan Low-Density Arrays, we identified 3 common miRNAs (miR-155, miR-23b, miR-27b) regulated by ITCs (sulforaphane, iberin) in colonic epithelial cell lines NCM460 and NCM356. In silico predictions allowed us to find 9 relevant single nucleotide polymorphisms (SNPs) localized within the 3UTRs of genes (AGTR1, TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs. Finally, we observed that homozygous CC genotype of DICER1, rs1057035, was significantly associated with decreased risk of CRC (odds ratio = 0.49; 95% confidence interval: 0.250.95, P = 0.036) when compared to TT homozygote genotype; also, the C allele tended to have a protective effect (P = 0.072). This study showed that miRNAs could be involved in chemoprotective effects of natural agents; their function alteration through SNPs in their binding sites and flanking regions presents a new class of CRC risk factors. Sporadic colorectal cancer (CRC) is a typical multifactorial disease. Isothiocyanates (ITC) have been recently shown to inhibit development of CRC in many experimental models. MicroRNAs (miRNAs) are short noncoding RNAs that posttranscriptionally regulate gene expression through binding to 3 untranslated regions (3UTR) of target mRNAs. MiRNAs are regulated by natural agents, ITCs included. In our study, using global expression profiling based on TaqMan Low-Density Arrays, we identified 3 common miRNAs (miR-155, miR-23b, miR-27b) regulated by ITCs (sulforaphane, iberin) in colonic epithelial cell lines NCM460 and NCM356. In silico predictions allowed us to find 9 relevant single nucleotide polymorphisms (SNPs) localized within the 3UTRs of genes (AGTR1, TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs. Finally, we observed that homozygous CC genotype of DICER1, rs1057035, was significantly associated with decreased risk of CRC (odds ratio = 0.49; 95% confidence interval: 0.250.95, P = 0.036) when compared to TT homozygote genotype; also, the C allele tended to have a protective effect (P = 0.072). This study showed that miRNAs could be involved in chemoprotective effects of natural agents; their function alteration through SNPs in their binding sites and flanking regions presents a new class of CRC risk factors.
dcterms:title
Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer
skos:prefLabel
Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer
skos:notation
RIV/00209805:_____/13:#0000472!RIV14-MZ0-00209805
n18:predkladatel
n19:ico%3A00209805
n4:aktivita
n10:P n10:Z
n4:aktivity
P(NS10352), Z(MZ0MOU2005)
n4:cisloPeriodika
2
n4:dodaniDat
n12:2014
n4:domaciTvurceVysledku
n6:3329100 n6:6760074 n6:2912589 n6:3116409 n6:8285721 n6:1863630 n6:5974291
n4:druhVysledku
n11:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
78689
n4:idVysledku
RIV/00209805:_____/13:#0000472
n4:jazykVysledku
n20:eng
n4:klicovaSlova
expression; cells; carcinoma; genotype; resource; broccoli; MIR-155; protein; growth; dicer
n4:klicoveSlovo
n5:broccoli n5:dicer n5:expression n5:protein n5:genotype n5:MIR-155 n5:cells n5:carcinoma n5:growth n5:resource
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[07F421BFD7AE]
n4:nazevZdroje
Nutrition and Cancer
n4:obor
n17:FD
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
11
n4:projekt
n9:NS10352
n4:rokUplatneniVysledku
n12:2013
n4:svazekPeriodika
65
n4:tvurceVysledku
Slabý, Ondřej Šachlová, Milana Vyzula, Rostislav Bischofová, Svatava Héžová, Renata Březková, Veronika Svoboda, Marek
n4:wos
000315352200010
n4:zamer
n7:MZ0MOU2005
s:issn
0163-5581
s:numberOfPages
8
n21:doi
10.1080/01635581.2013.756530