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Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F13%3A%230000415%21RIV14-GA0-00209805
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S1388248113001410
dcterms:description
Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and 2,2´-bipyridine, Os(VI)bipy, specifically binding to the 3´-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labelingstep with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture ofother noncomplementary miRNAs was possible. Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and 2,2´-bipyridine, Os(VI)bipy, specifically binding to the 3´-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labelingstep with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture ofother noncomplementary miRNAs was possible.
dcterms:title
Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers
skos:prefLabel
Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers
skos:notation
RIV/00209805:_____/13:#0000415!RIV14-GA0-00209805
n5:predkladatel
n16:ico%3A00209805
n3:aktivita
n7:Z n7:P n7:I
n3:aktivity
I, P(ED2.1.00/03.0101), P(GAP301/11/2055), P(GBP206/12/G151), Z(AV0Z50040702)
n3:cisloPeriodika
August
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n6:1330586 Bartošík, Martin n6:9637419 n6:9991646
n3:druhVysledku
n17:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
94808
n3:idVysledku
RIV/00209805:_____/13:#0000415
n3:jazykVysledku
n10:eng
n3:klicovaSlova
microRNA; cancer diagnostics; electrochemistry; mercury electrodes
n3:klicoveSlovo
n11:electrochemistry n11:microRNA n11:mercury%20electrodes n11:cancer%20diagnostics
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[BC8934C49E90]
n3:nazevZdroje
Electrochemistry Communications
n3:obor
n13:CG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n9:GAP301%2F11%2F2055 n9:ED2.1.00%2F03.0101 n9:GBP206%2F12%2FG151
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
33
n3:tvurceVysledku
Vojtěšek, Bořivoj Paleček, Emil Bartošík, Martin Hrstka, Roman
n3:wos
000323024600014
n3:zamer
n20:AV0Z50040702
s:issn
1388-2481
s:numberOfPages
4
n19:doi
10.1016/j.elecom.2013.04.009