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Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F13%3A%230000406%21RIV14-GA0-00209805
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
dcterms:description
The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ΔNp73 isoform likely has oncogenic potential. The expression of these isoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ΔNp73 in medulloblastoma cell lines. Due to a lack of suitable anti-ΔNp73 antibodies, we developed two new rabbit polyclonal antibodies, ΔNp73-26 and ΔNp73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the ΔNp73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the ΔNp73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the ΔNp73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with ΔNp73 and golgin-97, we showed the co-localization of the ΔNp73 isoform with the Golgi apparatus. Nevertheless, further detailed analyses of possible interactions of ΔNp73 with the proteins accumulated in the Golgi apparatus should be performed to explain the dynamics of ΔNp73 outside the cell nucleus. The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ΔNp73 isoform likely has oncogenic potential. The expression of these isoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ΔNp73 in medulloblastoma cell lines. Due to a lack of suitable anti-ΔNp73 antibodies, we developed two new rabbit polyclonal antibodies, ΔNp73-26 and ΔNp73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the ΔNp73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the ΔNp73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the ΔNp73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with ΔNp73 and golgin-97, we showed the co-localization of the ΔNp73 isoform with the Golgi apparatus. Nevertheless, further detailed analyses of possible interactions of ΔNp73 with the proteins accumulated in the Golgi apparatus should be performed to explain the dynamics of ΔNp73 outside the cell nucleus.
dcterms:title
Intracellular distribution of the ΔNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies Intracellular distribution of the ΔNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies
skos:prefLabel
Intracellular distribution of the ΔNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies Intracellular distribution of the ΔNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies
skos:notation
RIV/00209805:_____/13:#0000406!RIV14-GA0-00209805
n17:predkladatel
n18:ico%3A00209805
n3:aktivita
n13:P
n3:aktivity
P(ED2.1.00/03.0101), P(GAP301/11/2076)
n3:cisloPeriodika
7
n3:dodaniDat
n6:2014
n3:domaciTvurceVysledku
n12:3403149 n12:9637419
n3:druhVysledku
n9:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
80939
n3:idVysledku
RIV/00209805:_____/13:#0000406
n3:jazykVysledku
n10:eng
n3:klicovaSlova
medulloblastoma; DeltaNp73; immunocytochemistry; immunoblotting; transmission electron microscopy
n3:klicoveSlovo
n4:transmission%20electron%20microscopy n4:immunoblotting n4:DeltaNp73 n4:medulloblastoma n4:immunocytochemistry
n3:kodStatuVydavatele
ES - Španělské království
n3:kontrolniKodProRIV
[7D4DCBE60BA1]
n3:nazevZdroje
Histology and Histopathology
n3:obor
n15:FD
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
7
n3:projekt
n14:ED2.1.00%2F03.0101 n14:GAP301%2F11%2F2076
n3:rokUplatneniVysledku
n6:2013
n3:svazekPeriodika
28
n3:tvurceVysledku
Nekulová, Miroslava Vojtěšek, Bořivoj
n3:wos
000320710900011
s:issn
0213-3911
s:numberOfPages
12