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Statements

Subject Item
n2:RIV%2F00209805%3A_____%2F12%3A%230000340%21RIV13-MSM-00209805
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com.proxy.mzk.cz/doi/10.1111/j.1582-4934.2012.01579.x/abstract
dcterms:description
MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumour suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoural tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumour tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higher pre-operative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines. Similarly, overexpression of miR-375 and inhibition of miR-135b led to decreased viability. Finally, restoration of miR-378, miR-422a and miR-375 inhibited G1/S transition. These findings indicate that miR-378, miR-375, miR-422a and miR-215 play an important role in CRC as tumour suppressors, whereas miR-135b functions as an oncogene; both groups of miRNA contribute to CRC pathogenesis. MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumour suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoural tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumour tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higher pre-operative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines. Similarly, overexpression of miR-375 and inhibition of miR-135b led to decreased viability. Finally, restoration of miR-378, miR-422a and miR-375 inhibited G1/S transition. These findings indicate that miR-378, miR-375, miR-422a and miR-215 play an important role in CRC as tumour suppressors, whereas miR-135b functions as an oncogene; both groups of miRNA contribute to CRC pathogenesis.
dcterms:title
Identification and functional screening of microRNAs highly deregulated in colorectal cancer Identification and functional screening of microRNAs highly deregulated in colorectal cancer
skos:prefLabel
Identification and functional screening of microRNAs highly deregulated in colorectal cancer Identification and functional screening of microRNAs highly deregulated in colorectal cancer
skos:notation
RIV/00209805:_____/12:#0000340!RIV13-MSM-00209805
n6:aktivita
n11:Z n11:I n11:P
n6:aktivity
I, P(ED1.1.00/02.0068), P(LM2010004), P(NS9814), Z(MZ0MOU2005)
n6:cisloPeriodika
11
n6:dodaniDat
n9:2013
n6:domaciTvurceVysledku
n7:7274297 n7:6760074 n7:1012010 n7:5974291 n7:7947429 n7:2912589 n7:7835264
n6:druhVysledku
n13:J
n6:duvernostUdaju
n20:S
n6:entitaPredkladatele
n19:predkladatel
n6:idSjednocenehoVysledku
140265
n6:idVysledku
RIV/00209805:_____/12:#0000340
n6:jazykVysledku
n16:eng
n6:klicovaSlova
colorectal cancer; microRNA; expression profiling; apoptosis; migration
n6:klicoveSlovo
n8:expression%20profiling n8:apoptosis n8:microRNA n8:colorectal%20cancer n8:migration
n6:kodStatuVydavatele
US - Spojené státy americké
n6:kontrolniKodProRIV
[C3D19A015989]
n6:nazevZdroje
Journal of cellular and molecular medicine
n6:obor
n18:FD
n6:pocetDomacichTvurcuVysledku
7
n6:pocetTvurcuVysledku
12
n6:projekt
n14:ED1.1.00%2F02.0068 n14:NS9814 n14:LM2010004
n6:rokUplatneniVysledku
n9:2012
n6:svazekPeriodika
16
n6:tvurceVysledku
Vyzula, Rostislav Kiss, Igor Slabý, Ondřej Faltejsková, Petra Slabá, Kateřina Svoboda, Marek Fabian, Pavel
n6:wos
000310555200010
n6:zamer
n17:MZ0MOU2005
s:issn
1582-1838
s:numberOfPages
12
n4:doi
10.1111/j.1582-4934.2012.01579.x