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Statements

Subject Item
n2:RIV%2F00179906%3A_____%2F12%3A10124568%21RIV13-MZ0-00179906
rdf:type
n4:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1179/1607845412Y.0000000015
dcterms:description
Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL. Zeta-associated protein of 70 kDa (ZAP-70) is a tyrosine kinase that plays a role in signal transduction from the T-cell receptor. ZAP-70 is expressed in normal T-cells and NK-cells. Increased expression of ZAP-70 has been identified in chronic lymphocytic leukemia (CLL). CLL patients with increased ZAP-70 expression have significantly worse prognosis in terms of both progression-free survival and overall survival. There are several methods to quantify ZAP-70: polymerase chain reaction (PCR), immunoblotting, immunohistochemistry, and flow cytometry. Use of flow cytometry for ZAP-70 detection seems to be advantageous as this technique enables us to assess the presence of ZAP-70 separately on CLL clone, T-cells, and NK-cells. On the other hand, detection of ZAP-70 by flow cytometry is substantially influenced by many variables. The principal drawback of flow cytometry is the absence of consensus regarding selection of optimal anti-ZAP-70 antibody, fluorochrome conjugate, the most reliable staining technique, and optimal positivity threshold. This article summarizes pitfalls of flow cytometric analysis of ZAP-70 in CLL.
dcterms:title
Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia
skos:prefLabel
Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia
skos:notation
RIV/00179906:_____/12:10124568!RIV13-MZ0-00179906
n4:predkladatel
n19:ico%3A00179906
n5:aktivita
n8:I n8:P
n5:aktivity
I, P(NT13412)
n5:cisloPeriodika
5
n5:dodaniDat
n14:2013
n5:domaciTvurceVysledku
n7:7110847 n7:9106707 n7:8222525
n5:druhVysledku
n13:J
n5:duvernostUdaju
n16:S
n5:entitaPredkladatele
n20:predkladatel
n5:idSjednocenehoVysledku
158766
n5:idVysledku
RIV/00179906:_____/12:10124568
n5:jazykVysledku
n18:eng
n5:klicovaSlova
Standardization; Prognosis; Flow cytometry; CLL; ZAP-70
n5:klicoveSlovo
n12:Standardization n12:Prognosis n12:CLL n12:Flow%20cytometry n12:ZAP-70
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[BC1732549AC5]
n5:nazevZdroje
Hematology
n5:obor
n6:FN
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
3
n5:projekt
n11:NT13412
n5:rokUplatneniVysledku
n14:2012
n5:svazekPeriodika
17
n5:tvurceVysledku
Krejsek, Jan Smolej, Lukáš Vroblová, Vladimíra
n5:wos
000308350000004
s:issn
1024-5332
s:numberOfPages
7
n9:doi
10.1179/1607845412Y.0000000015