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Statements

Subject Item
n2:RIV%2F00179906%3A_____%2F11%3A10100325%21RIV12-MZ0-00179906
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0039914011005121
dcterms:description
A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice. A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
dcterms:title
Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC
skos:prefLabel
Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC
skos:notation
RIV/00179906:_____/11:10100325!RIV12-MZ0-00179906
n5:predkladatel
n6:ico%3A00179906
n3:aktivita
n9:Z n9:S
n3:aktivity
S, Z(MSM0021620820), Z(MSM0021620822), Z(MZ0FNHK2005)
n3:cisloPeriodika
3
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n19:3759032 n19:3703444 n19:3967158
n3:druhVysledku
n20:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
193853
n3:idVysledku
RIV/00179906:_____/11:10100325
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Clinical research; Monolithic column; HPLC; Tryptophan; Kynurenine; Neopterin
n3:klicoveSlovo
n7:Kynurenine n7:Tryptophan n7:Neopterin n7:Monolithic%20column n7:Clinical%20research n7:HPLC
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BF30932E48D9]
n3:nazevZdroje
Talanta
n3:obor
n17:FR
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
85
n3:tvurceVysledku
Kašparová, Markéta Solich, Petr Solichová, Dagmar Krčmová, Lenka Plíšek, Jiří Melichar, Bohuslav Sobotka, Luboš
n3:wos
000294092800038
n3:zamer
n12:MSM0021620820 n12:MZ0FNHK2005 n12:MSM0021620822
s:issn
0039-9140
s:numberOfPages
6
n18:doi
10.1016/j.talanta.2011.06.027