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Statements

Subject Item
n2:RIV%2F00159816%3A_____%2F14%3A00061040%21RIV15-MSM-00159816
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin(-)Sca-1(+) CPCs (iCPC(Sca-1)) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPC(Sca-1) were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPC(Sca-1) differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like cells when subjected to appropriate stimuli. The cell line consistently displayed features of Lin(-)Sca-1(+) CPCs in vitro, as well as in vivo after intramyocardial delivery in the onset of myocardial infarction (MI). Transplanted iCPC(Sca-1) significantly attenuated the functional and anatomical alterations caused by MI while promoting neovascularization. iCPC(Sca-1) are further shown to engraft, establish functional connections, and differentiate in loco into cardiomyocyte- and vasculature-like cells. These data validate iCPC(Sca-1) as an in vitro model system for Lin(-)Sca-1(+) progenitors and for systematic dissection of mechanisms underlying CPC subsets engraftment/differentiation in vivo. Moreover, iCPC(Sca-1) can be regarded as a ready-to-use CPCs source for pre-clinical bioengineering studies toward the development of novel strategies for restoration of the damaged myocardium. Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin(-)Sca-1(+) CPCs (iCPC(Sca-1)) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPC(Sca-1) were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPC(Sca-1) differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like cells when subjected to appropriate stimuli. The cell line consistently displayed features of Lin(-)Sca-1(+) CPCs in vitro, as well as in vivo after intramyocardial delivery in the onset of myocardial infarction (MI). Transplanted iCPC(Sca-1) significantly attenuated the functional and anatomical alterations caused by MI while promoting neovascularization. iCPC(Sca-1) are further shown to engraft, establish functional connections, and differentiate in loco into cardiomyocyte- and vasculature-like cells. These data validate iCPC(Sca-1) as an in vitro model system for Lin(-)Sca-1(+) progenitors and for systematic dissection of mechanisms underlying CPC subsets engraftment/differentiation in vivo. Moreover, iCPC(Sca-1) can be regarded as a ready-to-use CPCs source for pre-clinical bioengineering studies toward the development of novel strategies for restoration of the damaged myocardium.
dcterms:title
Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization
skos:prefLabel
Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization
skos:notation
RIV/00159816:_____/14:00061040!RIV15-MSM-00159816
n3:aktivita
n13:P
n3:aktivity
P(ED1.100/02/0123)
n3:cisloPeriodika
9
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
Forte, Giancarlo Pagliari, Stefania
n3:druhVysledku
n12:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
47000
n3:idVysledku
RIV/00159816:_____/14:00061040
n3:jazykVysledku
n16:eng
n3:klicovaSlova
cardiomyocytes; differentiation; life-span; mouse model; adult heart; ischemic-heart; stromal cells; telomerase expression; in-vitro model; side population cells
n3:klicoveSlovo
n5:telomerase%20expression n5:in-vitro%20model n5:adult%20heart n5:mouse%20model n5:side%20population%20cells n5:life-span n5:cardiomyocytes n5:ischemic-heart n5:differentiation n5:stromal%20cells
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[DDFF88EB56D7]
n3:nazevZdroje
Stem Cells and Development
n3:obor
n11:FD
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:projekt
n14:ED1.100%2F02%2F0123
n3:rokUplatneniVysledku
n15:2014
n3:svazekPeriodika
23
n3:tvurceVysledku
Nascimento, Diana S. Pagliari, Stefania Di Nardo, Paolo Resende, Tatiana P. Valente, Mariana Abreu, Claudia Freire, Ana G. Forte, Giancarlo Carvalho, Isabel Pinto-Do-O, Perpetua
n3:wos
000334845500009
s:issn
1547-3287
s:numberOfPages
15
n10:doi
10.1089/scd.2013.0305