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Statements

Subject Item
n2:RIV%2F00064211%3A_____%2F12%3A%230000209%21RIV14-MZ0-00064211
rdf:type
n14:Vysledek skos:Concept
dcterms:description
A large outbreak caused by a rare Shiga toxinproducing Escherichia coli serotype O104:H4 occurred in Germany in May to July 2011. The National Reference Laboratory for E. coli and Shigella investigated the stool sample from an American tourist with bloody diarrhea who arrived in the Czech Republic from Germany where she consumed salads with raw vegetable a week ago. Using culture of the enriched stool on extended-spectrum β- lactamase agar, we isolated E. coli strain which belonged to serotype O104:H4 as determined by conventional and molecular serotyping. The strain contained the major virulence characteristics of enterohemorrhagic E. coli (stx2 encoding Shiga toxin 2) and enteroaggregative E. coli (aggA encoding aggregative adherence fimbriae I). This unique combination of virulence traits demonstrated that this strain belongs to the hybrid enteroaggregative hemorrhagic E. coli clone which caused the German outbreak. Using advanced culture and molecular biological approaches is the prerequisite for identification of new, unusual pathogens. A large outbreak caused by a rare Shiga toxinproducing Escherichia coli serotype O104:H4 occurred in Germany in May to July 2011. The National Reference Laboratory for E. coli and Shigella investigated the stool sample from an American tourist with bloody diarrhea who arrived in the Czech Republic from Germany where she consumed salads with raw vegetable a week ago. Using culture of the enriched stool on extended-spectrum β- lactamase agar, we isolated E. coli strain which belonged to serotype O104:H4 as determined by conventional and molecular serotyping. The strain contained the major virulence characteristics of enterohemorrhagic E. coli (stx2 encoding Shiga toxin 2) and enteroaggregative E. coli (aggA encoding aggregative adherence fimbriae I). This unique combination of virulence traits demonstrated that this strain belongs to the hybrid enteroaggregative hemorrhagic E. coli clone which caused the German outbreak. Using advanced culture and molecular biological approaches is the prerequisite for identification of new, unusual pathogens.
dcterms:title
An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany
skos:prefLabel
An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany An imported case of bloody diarrhea in the Czech Republic caused by a hybrid enteroaggregative hemorrhhagic Escherichia coli (EAHEC) O104:H4 strain associated with the large outbreak in Germany
skos:notation
RIV/00064211:_____/12:#0000209!RIV14-MZ0-00064211
n14:predkladatel
n15:ico%3A00064211
n4:aktivita
n16:I
n4:aktivity
I
n4:cisloPeriodika
2
n4:dodaniDat
n11:2014
n4:domaciTvurceVysledku
n12:8820783 n12:2048140
n4:druhVysledku
n8:J
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n5:predkladatel
n4:idSjednocenehoVysledku
121974
n4:idVysledku
RIV/00064211:_____/12:#0000209
n4:jazykVysledku
n18:eng
n4:klicovaSlova
emorrhagic Eschericha coli; hemorrhagic diarrhea; hemolytic-uremic syndrom; shiga toxin
n4:klicoveSlovo
n7:hemolytic-uremic%20syndrom n7:hemorrhagic%20diarrhea n7:shiga%20toxin n7:emorrhagic%20Eschericha%20coli
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[B503EA6D582D]
n4:nazevZdroje
Folia microbiologica
n4:obor
n17:FN
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n11:2012
n4:svazekPeriodika
57
n4:tvurceVysledku
Petráš, Petr Marejková, Monika Reisingerová, Marie Roháčová, Hana
n4:wos
000303679900001
s:issn
0015-5632
s:numberOfPages
5
n13:doi
10.1007/s12223-011-0095-0