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Statements

Subject Item
n2:RIV%2F00064211%3A_____%2F11%3A%230000084%21RIV12-MZ0-00064211
rdf:type
skos:Concept n7:Vysledek
rdfs:seeAlso
http://www.springerlink.com/content/m204438557076672/
dcterms:description
Innate immune surveillance in the blood is executed mostly by circulating monocytes, which recognize conserved bacterial molecules such as peptidoglycan and lipopolysaccharide. Toll-like receptors (TLR) play a central role in microbe-associated molecular pattern detection. The aim of this study was to compare the differences in TLR expression and cytokine production after stimulation of peripheral blood cells with heat-killed gram-negative and gram-positive human pathogens: Neisseria meningitidis, Escherichia coli, Staphylococcus aureus, and Streptococcus pneumoniae. We found that TLR2 expression is up-regulated on monocytes after stimulation with S. aureus, S. pneumoniae, E. coli, and N. meningitidis. Moreover, TLR2 up-regulation was positively associated with increasing concentrations of gram-positive bacteria, whereas higher concentrations of gram-negative bacteria, especially E. coli, caused a milder TLR2 expression increase when compared to low doses. Cytokines were produced in similar dose-dependent profiles regardless of the stimulatory pathogen; however, gram-negative pathogens induced higher cytokine levels when compared to gram-positive bacteria at the same density. These results indicate that gram-positive and gram-negative bacteria differ in their dose-dependent patterns of induction of TLR2 and TLR4, but not cytokine expression Innate immune surveillance in the blood is executed mostly by circulating monocytes, which recognize conserved bacterial molecules such as peptidoglycan and lipopolysaccharide. Toll-like receptors (TLR) play a central role in microbe-associated molecular pattern detection. The aim of this study was to compare the differences in TLR expression and cytokine production after stimulation of peripheral blood cells with heat-killed gram-negative and gram-positive human pathogens: Neisseria meningitidis, Escherichia coli, Staphylococcus aureus, and Streptococcus pneumoniae. We found that TLR2 expression is up-regulated on monocytes after stimulation with S. aureus, S. pneumoniae, E. coli, and N. meningitidis. Moreover, TLR2 up-regulation was positively associated with increasing concentrations of gram-positive bacteria, whereas higher concentrations of gram-negative bacteria, especially E. coli, caused a milder TLR2 expression increase when compared to low doses. Cytokines were produced in similar dose-dependent profiles regardless of the stimulatory pathogen; however, gram-negative pathogens induced higher cytokine levels when compared to gram-positive bacteria at the same density. These results indicate that gram-positive and gram-negative bacteria differ in their dose-dependent patterns of induction of TLR2 and TLR4, but not cytokine expression
dcterms:title
Differences in Toll-like receptor expression and cytokine production after stimulation with heat-killed gram-positive and gram-negative bacteria Differences in Toll-like receptor expression and cytokine production after stimulation with heat-killed gram-positive and gram-negative bacteria
skos:prefLabel
Differences in Toll-like receptor expression and cytokine production after stimulation with heat-killed gram-positive and gram-negative bacteria Differences in Toll-like receptor expression and cytokine production after stimulation with heat-killed gram-positive and gram-negative bacteria
skos:notation
RIV/00064211:_____/11:#0000084!RIV12-MZ0-00064211
n7:predkladatel
n8:ico%3A00064211
n3:aktivita
n10:Z n10:S n10:P
n3:aktivity
P(NR9316), S, Z(MSM0021620806)
n3:cisloPeriodika
2
n3:dodaniDat
n11:2012
n3:domaciTvurceVysledku
n5:5649617 n5:8766002 n5:3039145
n3:druhVysledku
n16:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
194570
n3:idVysledku
RIV/00064211:_____/11:#0000084
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Toll-like receptor-2; Toll-like receptor-4; cytokines; Neisseria meningitidis; Escherichia coli; Staphylococcus aureus; Staphylococcus pneumonia
n3:klicoveSlovo
n4:Staphylococcus%20pneumonia n4:cytokines n4:Escherichia%20coli n4:Neisseria%20meningitidis n4:Staphylococcus%20aureus n4:Toll-like%20receptor-2 n4:Toll-like%20receptor-4
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[485E60249DAA]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n9:FN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n18:NR9316
n3:rokUplatneniVysledku
n11:2011
n3:svazekPeriodika
56
n3:tvurceVysledku
Potměšil, Roman Beran, Ondřej Holub, Michal
n3:wos
000291358600009
n3:zamer
n17:MSM0021620806
s:issn
0015-5632
s:numberOfPages
5
n15:doi
10.1007/s12223-011-0001-9