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Statements

Subject Item
n2:RIV%2F00064203%3A_____%2F12%3A8232%21RIV13-MZ0-00064203
rdf:type
n11:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1038/leu.2012.122
dcterms:description
The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database. The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
dcterms:title
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
skos:prefLabel
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
skos:notation
RIV/00064203:_____/12:8232!RIV13-MZ0-00064203
n11:predkladatel
n15:ico%3A00064203
n3:aktivita
n5:P n5:Z
n3:aktivity
P(NT12425), Z(MSM0021620813)
n3:cisloPeriodika
9
n3:dodaniDat
n6:2013
n3:domaciTvurceVysledku
n13:2780909 n13:8646597 n13:2842858
n3:druhVysledku
n20:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
134938
n3:idVysledku
RIV/00064203:_____/12:8232
n3:jazykVysledku
n7:eng
n3:klicovaSlova
flow cytometry; standardization; compensation; software; fluorochromes; immunophenotyping; minimal residual disease; chronic lymphoproliferative disorders; international consensus recommendations; acute lymphoblastic-leukemia; hematolymphoid neoplasia; hematologic neoplasia; medical indications; quality-control; compensation; reagents
n3:klicoveSlovo
n4:hematologic%20neoplasia n4:hematolymphoid%20neoplasia n4:minimal%20residual%20disease n4:fluorochromes n4:compensation n4:quality-control n4:software n4:medical%20indications n4:acute%20lymphoblastic-leukemia n4:flow%20cytometry n4:international%20consensus%20recommendations n4:immunophenotyping n4:reagents n4:standardization n4:chronic%20lymphoproliferative%20disorders
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[56D55CFBCC4D]
n3:nazevZdroje
Leukemia
n3:obor
n10:FD
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
21
n3:projekt
n18:NT12425
n3:rokUplatneniVysledku
n6:2012
n3:svazekPeriodika
26
n3:tvurceVysledku
Almeida, J. te Marvelde, JG Vidriales, MB Orfao, A. Perez, JJ Asnafi, V. Lhermitte, L. Szczepanski, T. Flores-Montero, J. Kalina, Tomáš Bottcher, S. Hrušák, Ondřej Cullen, M. van Dongen, JJM Mendonca, A. Martin-Ayuso, M. Mejstříková, Ester Ritgen, M. Sedek, L. de Tute, R. van der Velden, VHJ
n3:wos
000308342900004
n3:zamer
n17:MSM0021620813
s:issn
0887-6924
s:numberOfPages
25