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Statements

Subject Item
n2:RIV%2F00064203%3A_____%2F12%3A8216%21RIV13-MZ0-00064203
rdf:type
n9:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1088/0957-0233/23/10/105704
dcterms:description
Point-of-care (POC) systems require significant component integration to implement biochemical protocols associated with molecular diagnostic assays. Hybrid platforms where discrete components are combined in a single platform are a suitable approach to integration, where combining multiple device fabrication steps on a single substrate is not possible due to incompatible or costly fabrication steps. We integrate three devices each with a specific system functionality: (i) a silicon electro-wetting-on-dielectric (EWOD) device to move and mix sample and reagent droplets in an oil phase, (ii) a polymer microfluidic chip containing channels and reservoirs and (iii) an aqueous phase glass microarray for fluorescence microarray hybridization detection. The EWOD device offers the possibility of fully integrating on-chip sample preparation using nanolitre sample and reagent volumes. A key challenge is sample transfer from the oil phase EWOD device to the aqueous phase microarray for hybridization detection. The EWOD device, waveguide performance and functionality are maintained during the integration process. An on-chip biochemical protocol for arrayed primer extension (APEX) was implemented for single nucleotide polymorphism (SNiP) analysis. The prepared sample is aspirated from the EWOD oil phase to the aqueous phase microarray for hybridization. A bench-top instrumentation system was also developed around the integrated platform to drive the EWOD electrodes, implement APEX sample heating and image the microarray after hybridization. Point-of-care (POC) systems require significant component integration to implement biochemical protocols associated with molecular diagnostic assays. Hybrid platforms where discrete components are combined in a single platform are a suitable approach to integration, where combining multiple device fabrication steps on a single substrate is not possible due to incompatible or costly fabrication steps. We integrate three devices each with a specific system functionality: (i) a silicon electro-wetting-on-dielectric (EWOD) device to move and mix sample and reagent droplets in an oil phase, (ii) a polymer microfluidic chip containing channels and reservoirs and (iii) an aqueous phase glass microarray for fluorescence microarray hybridization detection. The EWOD device offers the possibility of fully integrating on-chip sample preparation using nanolitre sample and reagent volumes. A key challenge is sample transfer from the oil phase EWOD device to the aqueous phase microarray for hybridization detection. The EWOD device, waveguide performance and functionality are maintained during the integration process. An on-chip biochemical protocol for arrayed primer extension (APEX) was implemented for single nucleotide polymorphism (SNiP) analysis. The prepared sample is aspirated from the EWOD oil phase to the aqueous phase microarray for hybridization. A bench-top instrumentation system was also developed around the integrated platform to drive the EWOD electrodes, implement APEX sample heating and image the microarray after hybridization.
dcterms:title
A hybrid approach to device integration on a genetic analysis platform A hybrid approach to device integration on a genetic analysis platform
skos:prefLabel
A hybrid approach to device integration on a genetic analysis platform A hybrid approach to device integration on a genetic analysis platform
skos:notation
RIV/00064203:_____/12:8216!RIV13-MZ0-00064203
n9:predkladatel
n10:ico%3A00064203
n5:aktivita
n11:I
n5:aktivity
I
n5:cisloPeriodika
10
n5:dodaniDat
n14:2013
n5:domaciTvurceVysledku
n18:9104992
n5:druhVysledku
n13:J
n5:duvernostUdaju
n6:S
n5:entitaPredkladatele
n12:predkladatel
n5:idSjednocenehoVysledku
120257
n5:idVysledku
RIV/00064203:_____/12:8216
n5:jazykVysledku
n16:eng
n5:klicovaSlova
microfluidics; integration; EWOD; hybridization; dna hybridization; system; amplification; microchannel; droplets; chip; pcr
n5:klicoveSlovo
n7:droplets n7:microfluidics n7:integration n7:chip n7:hybridization n7:dna%20hybridization n7:system n7:microchannel n7:pcr n7:amplification n7:EWOD
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[30D573455A1F]
n5:nazevZdroje
Measurement Science & Technology
n5:obor
n17:EB
n5:pocetDomacichTvurcuVysledku
1
n5:pocetTvurcuVysledku
8
n5:rokUplatneniVysledku
n14:2012
n5:svazekPeriodika
23
n5:tvurceVysledku
Jary, D. Galvin, P. Aherne, M. Justice, J. Kurg, A. Berik, E. Brennan, D. Macek jr., Milan
n5:wos
000308920800047
s:issn
0957-0233
s:numberOfPages
8