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Statements

Subject Item
n2:RIV%2F00064203%3A_____%2F12%3A8159%21RIV13-MZ0-00064203
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1016/j.mcp.2012.04.001
dcterms:description
Background & aim: Hepatitis C is a liver disease caused by the hepatitis C virus. Interferon and ribavirin combination therapy has been a standard treatment of chronic hepatitis C. But only about 50% of patients have positive response to treatment and achieve so called sustained virological response. Recent studies indicate association of several single nucleotide polymorphisms near IL28B gene and response of hepatitis C patients to combined interferon/ribavirin treatment. In this study, rapid, specific and cost-effective small amplicon genotyping method for the two clinically important polymorphisms, rs12979860 C > T and rs8099917 T > G, near the IL28B gene is described. Methods: The distribution of genotypes of 181 HCV-uninfected Slovak Caucasians was analyzed using this novel method, based on a real-time melting analysis of the small amplicon. Results and conclusions: The frequency of wild-type (TT) homozygotes for rs8099917 was 66.30%, frequency of heterozygotes (TG) was 30.94% and we found only 2.76% subjects homozygous for risk G allele (allelic frequencies: T = 81.77%, G = 18.23%) were found. The frequency of wild-type genotype (CC) for rs12979860 was 49.72%, frequencies of heterozygous (CT) and risk-allele homozygous genotypes (TT) were 39.78% and 10.50%, respectively (allele frequencies: C = 69.61%, T = 30.39%). Statistically significant differences in the distribution of the alleles between the men and the women were not found. The novel method developed in our laboratory proved to be simple and highly customizable. Background & aim: Hepatitis C is a liver disease caused by the hepatitis C virus. Interferon and ribavirin combination therapy has been a standard treatment of chronic hepatitis C. But only about 50% of patients have positive response to treatment and achieve so called sustained virological response. Recent studies indicate association of several single nucleotide polymorphisms near IL28B gene and response of hepatitis C patients to combined interferon/ribavirin treatment. In this study, rapid, specific and cost-effective small amplicon genotyping method for the two clinically important polymorphisms, rs12979860 C > T and rs8099917 T > G, near the IL28B gene is described. Methods: The distribution of genotypes of 181 HCV-uninfected Slovak Caucasians was analyzed using this novel method, based on a real-time melting analysis of the small amplicon. Results and conclusions: The frequency of wild-type (TT) homozygotes for rs8099917 was 66.30%, frequency of heterozygotes (TG) was 30.94% and we found only 2.76% subjects homozygous for risk G allele (allelic frequencies: T = 81.77%, G = 18.23%) were found. The frequency of wild-type genotype (CC) for rs12979860 was 49.72%, frequencies of heterozygous (CT) and risk-allele homozygous genotypes (TT) were 39.78% and 10.50%, respectively (allele frequencies: C = 69.61%, T = 30.39%). Statistically significant differences in the distribution of the alleles between the men and the women were not found. The novel method developed in our laboratory proved to be simple and highly customizable.
dcterms:title
Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment
skos:prefLabel
Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment Closed tube method for rapid screening of IL28B polymorphisms involved in response to hepatitis C treatment
skos:notation
RIV/00064203:_____/12:8159!RIV13-MZ0-00064203
n12:predkladatel
n13:ico%3A00064203
n3:aktivita
n18:I
n3:aktivity
I
n3:cisloPeriodika
4
n3:dodaniDat
n5:2013
n3:domaciTvurceVysledku
n7:5597412
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
127446
n3:idVysledku
RIV/00064203:_____/12:8159
n3:jazykVysledku
n6:eng
n3:klicovaSlova
Hepatitis C; IL28B polymorphism; Small amplicon; Melting analysis; single-nucleotide polymorphisms; melting analysis; ribavirin therapy; genetic-variation; interferon; clearance; virus
n3:klicoveSlovo
n4:clearance n4:ribavirin%20therapy n4:melting%20analysis n4:single-nucleotide%20polymorphisms n4:interferon n4:Hepatitis%20C n4:Melting%20analysis n4:IL28B%20polymorphism n4:Small%20amplicon n4:virus n4:genetic-variation
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[CF2879DF86DC]
n3:nazevZdroje
Molecular and Cellular Probes
n3:obor
n8:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n5:2012
n3:svazekPeriodika
26
n3:tvurceVysledku
Habalova, V. Valkova, I. Zidzik, J. Schreter, I. Salagovic, J. Vasovčák, Peter Kristian, P. Klimcakova, L.
n3:wos
000305658700003
s:issn
0890-8508
s:numberOfPages
5