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Statements

Subject Item
n2:RIV%2F00064203%3A_____%2F10%3A6272%21RIV11-MZ0-00064203
rdf:type
n6:Vysledek skos:Concept
dcterms:description
We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument. We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument.
dcterms:title
Zeptomole Electrochemical Detection of Metallothioneins Zeptomole Electrochemical Detection of Metallothioneins
skos:prefLabel
Zeptomole Electrochemical Detection of Metallothioneins Zeptomole Electrochemical Detection of Metallothioneins
skos:notation
RIV/00064203:_____/10:6272!RIV11-MZ0-00064203
n4:aktivita
n14:I n14:P
n4:aktivity
I, P(IAA401990701)
n4:cisloPeriodika
7
n4:dodaniDat
n8:2011
n4:domaciTvurceVysledku
n15:9974687
n4:druhVysledku
n10:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
299929
n4:idVysledku
RIV/00064203:_____/10:6272
n4:jazykVysledku
n12:eng
n4:klicovaSlova
plasma-mass spectrometry; cancer; chronopotentiometry; speciation; proteomics; proteins; level
n4:klicoveSlovo
n13:cancer n13:plasma-mass%20spectrometry n13:chronopotentiometry n13:proteins n13:proteomics n13:level n13:speciation
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[ECC53BD87802]
n4:nazevZdroje
PLoS One
n4:obor
n9:FD
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
6
n4:projekt
n11:IAA401990701
n4:rokUplatneniVysledku
n8:2010
n4:svazekPeriodika
5
n4:tvurceVysledku
Wang, J. Kizek, R. Eckschlager, Tomáš Petrlova, J. Adam, V. Trnková, L.
n4:wos
000279522500009
s:issn
1932-6203
s:numberOfPages
8