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Statements

Subject Item
n2:RIV%2F00064173%3A_____%2F10%3A00002449%21RIV13-MZ0-00064173
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study. Experimental Dermatology 2010. Abstract Introduction: Monitoring of circulating melanoma cells in the peripheral blood is a promising method for identifying a subgroup of patients with minimal residual disease. Objectives: To evaluate the prognostic impact of melanoma-associated antigens by multimarker real-time RT-PCR for disease-specific survival time. Methods: Five melanoma markers: Melan-A, gp100, MAGE-3, MIA and tyrosinase were detected by a quantitative multimarker real-time reverse transcription-PCR (RT-PCR). We included 65 patients with resected melanoma in stage II-III. Peripheral blood samples were examined every 3 months for 2 years. The expression of melanoma markers in 2925 RT-PCR assays was correlated with clinical staging results in total of 5 years. Results: Twenty-seven patients relapsed during the study period and 26 of them revealed positive markers. MAGE-3 was the most sensitive progression marker in single occurrence or in combination with MIA and gp100. The time distribution of metastases during the screened period was as follows: progression in the first year was observed in 40.7% patients, second year in 25.9%, third year in 18.6%, fourth and fifth year in 7.4% equally. Conclusions: Statistically significant tumor marker elevation during the first 2 years after the surgical treatment correlates with a worse prognosis of patients. In contrast, the group showing negative real-time RT-PCR results in 24 months serial blood testing was associated with prolonged 5-year disease-specific survival. Therefore, quantitative detection of melanoma-specific molecular markers in the presented setting represents a useful tool for selecting patients in a higher risk of disease recurrence Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study. Experimental Dermatology 2010. Abstract Introduction: Monitoring of circulating melanoma cells in the peripheral blood is a promising method for identifying a subgroup of patients with minimal residual disease. Objectives: To evaluate the prognostic impact of melanoma-associated antigens by multimarker real-time RT-PCR for disease-specific survival time. Methods: Five melanoma markers: Melan-A, gp100, MAGE-3, MIA and tyrosinase were detected by a quantitative multimarker real-time reverse transcription-PCR (RT-PCR). We included 65 patients with resected melanoma in stage II-III. Peripheral blood samples were examined every 3 months for 2 years. The expression of melanoma markers in 2925 RT-PCR assays was correlated with clinical staging results in total of 5 years. Results: Twenty-seven patients relapsed during the study period and 26 of them revealed positive markers. MAGE-3 was the most sensitive progression marker in single occurrence or in combination with MIA and gp100. The time distribution of metastases during the screened period was as follows: progression in the first year was observed in 40.7% patients, second year in 25.9%, third year in 18.6%, fourth and fifth year in 7.4% equally. Conclusions: Statistically significant tumor marker elevation during the first 2 years after the surgical treatment correlates with a worse prognosis of patients. In contrast, the group showing negative real-time RT-PCR results in 24 months serial blood testing was associated with prolonged 5-year disease-specific survival. Therefore, quantitative detection of melanoma-specific molecular markers in the presented setting represents a useful tool for selecting patients in a higher risk of disease recurrence
dcterms:title
Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study
skos:prefLabel
Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study Quantitative detection of melanoma-associated antigens by multimarker real-time RT-PCR for molecular staging: results of a 5 years study
skos:notation
RIV/00064173:_____/10:00002449!RIV13-MZ0-00064173
n5:aktivita
n14:V n14:P
n5:aktivity
P(1A8243), V
n5:cisloPeriodika
11
n5:dodaniDat
n13:2013
n5:domaciTvurceVysledku
n9:6628885 n9:6463754 n9:5647797
n5:druhVysledku
n6:J
n5:duvernostUdaju
n10:S
n5:entitaPredkladatele
n11:predkladatel
n5:idSjednocenehoVysledku
283625
n5:idVysledku
RIV/00064173:_____/10:00002449
n5:jazykVysledku
n17:eng
n5:klicovaSlova
real-time RT-PCR; malignant melanoma; detection; circulating melanoma cells
n5:klicoveSlovo
n12:malignant%20melanoma n12:circulating%20melanoma%20cells n12:real-time%20RT-PCR n12:detection
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[2EC5B60C44BD]
n5:nazevZdroje
Experimental Dermatology
n5:obor
n18:FO
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
4
n5:projekt
n7:1A8243
n5:rokUplatneniVysledku
n13:2010
n5:svazekPeriodika
19
n5:tvurceVysledku
Arenberger, Petr Arenbergerová, Monika Gkalpakiotis, Spyridon
n5:wos
000283373800007
s:issn
0906-6705
s:numberOfPages
6
n16:doi
10.1111/j.1600-0625.2010.01123.x