This HTML5 document contains 42 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n18http://linked.opendata.cz/resource/domain/vavai/projekt/
n4http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n8http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
n9http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00064173%3A_____%2F09%3A00001928%21RIV13-MZ0-00064173/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n14http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n17http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n16http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00064173%3A_____%2F09%3A00001928%21RIV13-MZ0-00064173
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The aim of our study was to detect the influence of high-pressure treatment (HPT) on the allergenicity of recombinant allergen rDau c1 and carrot juice using in vitro and in vivo tests. The buffer solution of recombinant main carrot allergen rDau c1 for the basophil activation test (BAT) and Western blot (WB) was used. This pressure procedure did not influence the immune reactivity of rDau c1 in WB test. The structural changes of rDau c1 caused by HPT studied by circular dichroismus spectra were found. Mild increase of the beta-sheet structure was observed. The main changes were seen in rDau c1 samples treated 10 min at 500 MPa and temperature 50 °C. The influence of HPT on the allergenicity of carrot juice was studied afterward. The reactivity in skin prick tests and BAT did not show any influence of HPT on allergenicity of carrot juice. Pressure of 500 MPa for 10 min and temperature 30 °C, 40 °C, and 50 °C did not inactivate allergen Dau c1 in carrot juice in WB. HPT from 450 to 550 MPa for 3 and 10 min at temperature 30 °C had no influence on the immune reactivity of Dau c1 in carrot juice. Nineteen patients underwent the double-blind, placebo-controlled food challenge. Thirteen of them reacted to placebo and were excluded, one patient did not react at any material (placebo, HPT material and fresh frozen carrot juice), three patients had positive test (reacted on HPT material and non-treated fresh frozen carrot juice), and two patients had negative reaction (reacted only on fresh frozen material). We could not confirm the influence of HPT on allergenicity of rDau c1 and carrot juice using in vitro and in vivo tests. The aim of our study was to detect the influence of high-pressure treatment (HPT) on the allergenicity of recombinant allergen rDau c1 and carrot juice using in vitro and in vivo tests. The buffer solution of recombinant main carrot allergen rDau c1 for the basophil activation test (BAT) and Western blot (WB) was used. This pressure procedure did not influence the immune reactivity of rDau c1 in WB test. The structural changes of rDau c1 caused by HPT studied by circular dichroismus spectra were found. Mild increase of the beta-sheet structure was observed. The main changes were seen in rDau c1 samples treated 10 min at 500 MPa and temperature 50 °C. The influence of HPT on the allergenicity of carrot juice was studied afterward. The reactivity in skin prick tests and BAT did not show any influence of HPT on allergenicity of carrot juice. Pressure of 500 MPa for 10 min and temperature 30 °C, 40 °C, and 50 °C did not inactivate allergen Dau c1 in carrot juice in WB. HPT from 450 to 550 MPa for 3 and 10 min at temperature 30 °C had no influence on the immune reactivity of Dau c1 in carrot juice. Nineteen patients underwent the double-blind, placebo-controlled food challenge. Thirteen of them reacted to placebo and were excluded, one patient did not react at any material (placebo, HPT material and fresh frozen carrot juice), three patients had positive test (reacted on HPT material and non-treated fresh frozen carrot juice), and two patients had negative reaction (reacted only on fresh frozen material). We could not confirm the influence of HPT on allergenicity of rDau c1 and carrot juice using in vitro and in vivo tests.
dcterms:title
Influence of high-pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests Influence of high-pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests
skos:prefLabel
Influence of high-pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests Influence of high-pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests
skos:notation
RIV/00064173:_____/09:00001928!RIV13-MZ0-00064173
n3:aktivita
n10:P n10:V
n3:aktivity
P(2B06139), V
n3:cisloPeriodika
4
n3:dodaniDat
n16:2013
n3:domaciTvurceVysledku
n4:6994121 n4:9360344
n3:druhVysledku
n7:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
319239
n3:idVysledku
RIV/00064173:_____/09:00001928
n3:jazykVysledku
n13:eng
n3:klicovaSlova
high-pressure treatment; allergen; Dau c1; carrot
n3:klicoveSlovo
n17:allergen n17:high-pressure%20treatment n17:carrot n17:Dau%20c1
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[11F35246084D]
n3:nazevZdroje
High Pressure Research
n3:obor
n15:EC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:projekt
n18:2B06139
n3:rokUplatneniVysledku
n16:2009
n3:svazekPeriodika
29
n3:tvurceVysledku
Kučera, Petr Vávrová, Helena
n3:wos
000274675700043
s:issn
0895-7959
s:numberOfPages
10
n14:doi
10.1080/08957950903481176