This HTML5 document contains 46 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n10http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n17http://linked.opendata.cz/resource/domain/vavai/subjekt/
n16http://linked.opendata.cz/ontology/domain/vavai/
n18http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n13http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00064165%3A_____%2F11%3A10223%21RIV12-MZ0-00064165/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n15http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00064165%3A_____%2F11%3A10223%21RIV12-MZ0-00064165
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Mitochondrial production of reactive oxygen species (ROS) due to up-regulated glucose oxidation is thought to play a crucial, unifying role in the pathogenesis of chronic complications associated with diabetes mellitus. Mitochondrial permeability transition (MPT) is an interesting phenomenon involved in calcium signalling and cell death. We investigated the effects of glucose and several of its metabolites on calcium-induced MPT (measured as mitochondrial swelling) in isolated rat liver mitochondria. The presence of glucose, glucose 1-phosphate (both at 30 mM) or methylglyoxal (6 mM) significantly slowed calcium-induced mitochondria! swelling. Thirty mM glucose also resulted in a significant delay of MPT onset. In contrast, 30 mM fructose 6-phosphate accelerated swelling, whereas glucose 6-phosphate did not influence the MPT. The calcium binding potentials of the three hexose phosphates were tested and found similar. in vitro hydrogen peroxide production by mitochondria respiring on succinate in the presence of rotenone was independent of mitochondrial membrane potential and increased transiently during calcium-induced MPT. Inhibition of MPT with cyclosporine A resulted in decreased mitochondrial ROS production in response to calcium. In contrast, inhibition of MPT by methylglyoxal was accompanied by increased ROS production in response to calcium. In conclusion, we confirm that methylglyoxal is a potent inhibitor of MPT. In addition, high levels of glucose, glucose 1-phosphate and fructose 6-phosphate can also affect MPT. Methylglyoxal simultaneously inhibits MPT and increases mitochondrial ROS production in response to calcium. Our findings provide a novel context for the role of MPT in glucose sensing and the cellular toxicity caused by methylglyoxal. Mitochondrial production of reactive oxygen species (ROS) due to up-regulated glucose oxidation is thought to play a crucial, unifying role in the pathogenesis of chronic complications associated with diabetes mellitus. Mitochondrial permeability transition (MPT) is an interesting phenomenon involved in calcium signalling and cell death. We investigated the effects of glucose and several of its metabolites on calcium-induced MPT (measured as mitochondrial swelling) in isolated rat liver mitochondria. The presence of glucose, glucose 1-phosphate (both at 30 mM) or methylglyoxal (6 mM) significantly slowed calcium-induced mitochondria! swelling. Thirty mM glucose also resulted in a significant delay of MPT onset. In contrast, 30 mM fructose 6-phosphate accelerated swelling, whereas glucose 6-phosphate did not influence the MPT. The calcium binding potentials of the three hexose phosphates were tested and found similar. in vitro hydrogen peroxide production by mitochondria respiring on succinate in the presence of rotenone was independent of mitochondrial membrane potential and increased transiently during calcium-induced MPT. Inhibition of MPT with cyclosporine A resulted in decreased mitochondrial ROS production in response to calcium. In contrast, inhibition of MPT by methylglyoxal was accompanied by increased ROS production in response to calcium. In conclusion, we confirm that methylglyoxal is a potent inhibitor of MPT. In addition, high levels of glucose, glucose 1-phosphate and fructose 6-phosphate can also affect MPT. Methylglyoxal simultaneously inhibits MPT and increases mitochondrial ROS production in response to calcium. Our findings provide a novel context for the role of MPT in glucose sensing and the cellular toxicity caused by methylglyoxal.
dcterms:title
Glucose and Its Metabolites Have Distinct Effects on the Calcium-Induced Mitochondrial Permeability Transition Glucose and Its Metabolites Have Distinct Effects on the Calcium-Induced Mitochondrial Permeability Transition
skos:prefLabel
Glucose and Its Metabolites Have Distinct Effects on the Calcium-Induced Mitochondrial Permeability Transition Glucose and Its Metabolites Have Distinct Effects on the Calcium-Induced Mitochondrial Permeability Transition
skos:notation
RIV/00064165:_____/11:10223!RIV12-MZ0-00064165
n16:predkladatel
n17:ico%3A00064165
n4:aktivita
n8:I n8:S n8:Z
n4:aktivity
I, S, Z(MSM0021620807)
n4:cisloPeriodika
3
n4:dodaniDat
n15:2012
n4:domaciTvurceVysledku
n10:7171595
n4:druhVysledku
n7:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
201375
n4:idVysledku
RIV/00064165:_____/11:10223
n4:jazykVysledku
n6:eng
n4:klicovaSlova
mitochondrial permeability transition pore; diabetes mellitus; oxidative stress; glycolysis; methylglyoxal
n4:klicoveSlovo
n9:glycolysis n9:methylglyoxal n9:oxidative%20stress n9:diabetes%20mellitus n9:mitochondrial%20permeability%20transition%20pore
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[2A752247907B]
n4:nazevZdroje
Folia Biologica
n4:obor
n12:CE
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:rokUplatneniVysledku
n15:2011
n4:svazekPeriodika
57
n4:tvurceVysledku
Pláteník, Jan Buchal, Richard Sedláčková, Eva Gáll, Juraj Škrha, Jan jr.
n4:wos
000292574800002
n4:zamer
n18:MSM0021620807
s:issn
0015-5500
s:numberOfPages
8