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Statements

Subject Item
n2:RIV%2F00027162%3A_____%2F14%3A%230001151%21RIV15-MZE-00027162
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Methods and ResultsTwo virus strains from each of six animal virus families, including both enveloped and nonenveloped viruses, were lyophilized in (i) culture medium, (ii) with the addition of gelatine-sucrose and (iii) skim milk-sodium glutamate. All the virus suspensions were frozen (i) at -80 degrees C or (ii) in liquid nitrogen before lyophilization. Virus titre assay after lyophilization and after 8months storage at 4 degrees C revealed that the efficacy of stabilizers depended on virus structure. Generally, the best protective quality for enveloped viruses was achieved with gelatine-sucrose, which best maintained their infectivity and envelope morphology. Even additive-free culture medium proved adequate for nonenveloped viruses. Differences in stabilizer efficacy were also found between virus families and were expressed immediately after lyophilization; the activity of stabilizers in the course of storage was very similar. Freezing in liquid nitrogen proved beneficial for picornaviruses. ConclusionsThe choice of an appropriate stabilizer with respect to virus type is crucial for effective lyophilization. Significance and Impact of the StudyThis study contributes to the establishment of general guidelines for animal virus lyophilization, with particular respect to differences in virus structure. Methods and ResultsTwo virus strains from each of six animal virus families, including both enveloped and nonenveloped viruses, were lyophilized in (i) culture medium, (ii) with the addition of gelatine-sucrose and (iii) skim milk-sodium glutamate. All the virus suspensions were frozen (i) at -80 degrees C or (ii) in liquid nitrogen before lyophilization. Virus titre assay after lyophilization and after 8months storage at 4 degrees C revealed that the efficacy of stabilizers depended on virus structure. Generally, the best protective quality for enveloped viruses was achieved with gelatine-sucrose, which best maintained their infectivity and envelope morphology. Even additive-free culture medium proved adequate for nonenveloped viruses. Differences in stabilizer efficacy were also found between virus families and were expressed immediately after lyophilization; the activity of stabilizers in the course of storage was very similar. Freezing in liquid nitrogen proved beneficial for picornaviruses. ConclusionsThe choice of an appropriate stabilizer with respect to virus type is crucial for effective lyophilization. Significance and Impact of the StudyThis study contributes to the establishment of general guidelines for animal virus lyophilization, with particular respect to differences in virus structure.
dcterms:title
The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage
skos:prefLabel
The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage The influence of stabilizers and rates of freezing on preserving of structurally different animal viruses during lyophilization and subsequent storage
skos:notation
RIV/00027162:_____/14:#0001151!RIV15-MZE-00027162
n3:aktivita
n7:Z n7:P
n3:aktivity
P(LO1218), Z(MZE0002716202)
n3:cisloPeriodika
6
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n10:7925158
n3:druhVysledku
n9:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
21614
n3:idVysledku
RIV/00027162:_____/14:#0001151
n3:jazykVysledku
n18:eng
n3:klicovaSlova
animal virus lyophilization; animal virus preservation; freezing rate; stabilizer; virus structure
n3:klicoveSlovo
n13:stabilizer n13:freezing%20rate n13:virus%20structure n13:animal%20virus%20lyophilization n13:animal%20virus%20preservation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[9ED4E7E451F7]
n3:nazevZdroje
Journal of Applied Microbiology
n3:obor
n19:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:projekt
n8:LO1218
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
117
n3:tvurceVysledku
Malenovská, Hana
n3:wos
000345510300026
n3:zamer
n15:MZE0002716202
s:issn
1364-5072
s:numberOfPages
10
n17:doi
10.1111/jam.12654